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Applied and Environmental Microbiology, August 1999, p. 3325-3327, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Spheroplast Lysis Assay for Yeast in Microtiter Plate Format

Rafael Ovalle, Moyah Spencer, Monthiwa Thiwanont, and Peter N. Lipke*

Department of Biological Sciences and Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York, New York 10021

Received 9 February 1999/Accepted 18 May 1999

A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for drug susceptibility and for analyzing mutant phenotypes.

* Corresponding author. Mailing address: Department of Biological Sciences, Hunter College, 695 Park Avenue, New York, NY 10021. Phone: (212) 772-5235. Fax: (212) 772-5227. E-mail: lipke{at}genectr.hunter.cuny.edu.

Applied and Environmental Microbiology, August 1999, p. 3325-3327, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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