AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Steenkamp, E. T.
Right arrow Articles by Marasas, W. F. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steenkamp, E. T.
Right arrow Articles by Marasas, W. F. O.
Agricola
Right arrow Articles by Steenkamp, E. T.
Right arrow Articles by Marasas, W. F. O.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 1999, p. 3401-3406, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Differentiation of Fusarium subglutinans f. sp. pini by Histone Gene Sequence Data

E. T. Steenkamp,1,* B. D. Wingfield,1 T. A. Coutinho,1 M. J. Wingfield,1 and W. F. O. Marasas2

Tree Pathology Co-operative Programme, Forestry and Agricultural Biotechnology Institute, Departments of Genetics, Microbiology and Plant Pathology, University of Pretoria, Pretoria 0002,1 and Programme on Mycotoxins and Experimental Carcinogenesis, Medical Research Council, Tygerberg 7050,2 South Africa

Received 9 February 1999/Accepted 28 May 1999

Fusarium subglutinans f. sp. pini (= F. circinatum) is a pathogen of pine and is one of eight mating populations (i.e., biological species) in the Gibberella fujikuroi species complex. This species complex includes F. thapsinum, F. moniliforme (= F. verticillioides), F. nygamai, and F. proliferatum, as well as F. subglutinans associated with sugarcane, maize, mango, and pineapple. Differentiating these forms of F. subglutinans usually requires pathogenicity tests, which are often time-consuming and inconclusive. Our objective was to develop a technique to differentiate isolates of F. subglutinans f. sp. pini from other isolates identified as F. subglutinans. We sequenced the histone H3 gene from a representative set of Fusarium isolates. The H3 gene sequence was conserved and contained two introns in all the isolates studied. From both the intron and the exon sequence data, we developed a PCR-restriction fragment length polymorphism technique that reliably distinguishes F. subglutinans f. sp. pini from the other biological species in the G. fujikuroi species complex.

* Corresponding author. Mailing address: Tree Pathology Co-operative Programme, Forestry and Agricultural Biotechnology Institute, University of Pretoria, 74 Lunnon Rd., Hillcrest, Pretoria 0002, South Africa. Phone: (27 12) 420-3948. Fax: (27 12) 420-3947. E-mail: emma.steenkamp{at}fabi.up.ac.za.

Applied and Environmental Microbiology, August 1999, p. 3401-3406, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.