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Applied and Environmental Microbiology, August 1999, p. 3413-3417, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Karyotype of the White Rot Fungus Pleurotus ostreatus

Luis M. Larraya,1 Gumer Pérez,1 María M. Peñas,1 Johan J. P. Baars,2 Thomas S. P. Mikosch,2 Antonio G. Pisabarro,1 and Lucía Ramírez1,*

Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain,1 and Mushroom Experimental Station, NL-5960 AA Horst, The Netherlands2

Received 8 February 1999/Accepted 26 May 1999

The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure. In this study, we analyzed the genomic constitution of P. ostreatus by using pulsed-field gel electrophoresis optimized for the separation of its chromosomes. We have determined that it contains 11 pairs of chromosomes with sizes ranging from 1.4 to 4.7 Mbp. In addition to chromosome separation, the use of single-copy DNA probes allowed us to resolve the ambiguities caused by chromosome comigration. When the two nuclei present in the dikaryon were separated by protoplasting, analysis of their karyotypes revealed length polymorphisms affecting various chromosomes. This is, to our knowledge, the clearest chromosome separation available for this species.

* Corresponding author. Mailing address: Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain. Phone: (34) 948 169 130. Fax: (34) 948 169 732. E-mail: lramirez{at}upna.es.

Applied and Environmental Microbiology, August 1999, p. 3413-3417, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.