This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rosencrantz, D.
Right arrow Articles by Janssen, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rosencrantz, D.
Right arrow Articles by Janssen, P. H.
Agricola
Right arrow Articles by Rosencrantz, D.
Right arrow Articles by Janssen, P. H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 1999, p. 3526-3533, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Culturable Populations of Sporomusa spp. and Desulfovibrio spp. in the Anoxic Bulk Soil of Flooded Rice Microcosms

Dirk Rosencrantz,1 Frederick A. Rainey,2,dagger and Peter H. Janssen1,*

Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg,1 and Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-38124 Braunschweig,2 Germany

Received 2 February 1999/Accepted 18 May 1999

Most-probable-number (MPN) counts were made of homoacetogenic and other bacteria present in the anoxic flooded bulk soil of laboratory microcosms containing 90- to 95-day-old rice plants. MPN counts with substrates known to be useful for the selective enrichment or the cultivation of homoacetogenic bacteria (betaine, ethylene glycol, 2,3-butanediol, and 3,4,5-trimethoxybenzoate) gave counts of 2.3 × 103 to 2.8 × 105 cells per g of dry soil. Homoacetogens isolated from the terminal positive steps of these dilution cultures belonged to the genus Sporomusa. Counts with succinate, ethanol, and lactate gave much higher MPNs of 5.9 × 105 to 3.4 × 107 cells per g of dry soil and led to the isolation of Desulfovibrio spp. Counting experiments on lactate and ethanol which included Methanospirillum hungatei in the medium gave MPNs of 2.3 × 106 to 7.5 × 108 cells per g of dry soil and led to the isolation of Sporomusa spp. The latter strains could grow with betaine, ethylene glycol, 2,3-butanediol, and/or 3,4,5-trimethoxybenzoate, but apparently most cells of Sporomusa spp. did not initiate growth in counting experiments with those substrates. Spores apparently accounted for 2.2% or less of the culturable bacteria. It appears that culturable Desulfovibrio spp. and Sporomusa spp. were present in approximately equal numbers in the bulk soil. Multiple, phylogenetically-distinct, phenotypically-different, strains of each genus were found in the same soil system.

* Corresponding author. Present address: Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052, Australia. Phone: 61(3) 9344 5706. Fax: 61 (3) 9347 1540. E-mail: p.janssen{at}microbiology.unimelb.edu.au.

dagger Present address: Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803.

Applied and Environmental Microbiology, August 1999, p. 3526-3533, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Brodie, E. L., DeSantis, T. Z., Joyner, D. C., Baek, S. M., Larsen, J. T., Andersen, G. L., Hazen, T. C., Richardson, P. M., Herman, D. J., Tokunaga, T. K., Wan, J. M., Firestone, M. K. (2006). Application of a High-Density Oligonucleotide Microarray Approach To Study Bacterial Population Dynamics during Uranium Reduction and Reoxidation. Appl. Environ. Microbiol. 72: 6288-6298 [Abstract] [Full Text]  
  • Akasaka, H., Ueki, A., Hanada, S., Kamagata, Y., Ueki, K. (2003). Propionicimonas paludicola gen. nov., sp. nov., a novel facultatively anaerobic, Gram-positive, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil. Int. J. Syst. Evol. Microbiol. 53: 1991-1998 [Abstract] [Full Text]  
  • Boga, H. I., Ludwig, W., Brune, A. (2003). Sporomusa aerivorans sp. nov., an oxygen-reducing homoacetogenic bacterium from the gut of a soil-feeding termite. Int. J. Syst. Evol. Microbiol. 53: 1397-1404 [Abstract] [Full Text]  
  • Janssen, P. H., Yates, P. S., Grinton, B. E., Taylor, P. M., Sait, M. (2002). Improved Culturability of Soil Bacteria and Isolation in Pure Culture of Novel Members of the Divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. Appl. Environ. Microbiol. 68: 2391-2396 [Abstract] [Full Text]  
  • Chin, K.-J., Janssen, P. H. (2002). Propionate Formation by Opitutus terrae in Pure Culture and in Mixed Culture with a Hydrogenotrophic Methanogen and Implications for Carbon Fluxes in Anoxic Rice Paddy Soil. Appl. Environ. Microbiol. 68: 2089-2092 [Abstract] [Full Text]  
  • Conrad, R., Klose, M., Claus, P. (2000). Phosphate Inhibits Acetotrophic Methanogenesis on Rice Roots. Appl. Environ. Microbiol. 66: 828-831 [Abstract] [Full Text]  
  • Chin, K.-J., Hahn, D., Hengstmann, U., Liesack, W., Janssen, P. H. (1999). Characterization and Identification of Numerically Abundant Culturable Bacteria from the Anoxic Bulk Soil of Rice Paddy Microcosms. Appl. Environ. Microbiol. 65: 5042-5049 [Abstract] [Full Text]  
  • Hengstmann, U., Chin, K.-J., Janssen, P. H., Liesack, W. (1999). Comparative Phylogenetic Assignment of Environmental Sequences of Genes Encoding 16S rRNA and Numerically Abundant Culturable Bacteria from an Anoxic Rice Paddy Soil. Appl. Environ. Microbiol. 65: 5050-5058 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»