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Applied and Environmental Microbiology, August 1999, p. 3717-3720, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development and Testing of a Microbiological Assay To Detect Residual Effects of Disinfectant on Hard Surfaces

Alberto Mariscal,* Manuel Carnero-Varo, Jorge Gómez-Aracena, and Joaquín Fernández-Crehuet

Department of Preventive Medicine and Public Health, Facultad de Medicina, Universidad de Málaga, E-29071 Málaga, Spain

Received 11 February 1999/Accepted 24 May 1999

We describe a glucuronidase bioassay for detecting residual bactericidal activity from the use of disinfectants on hard surfaces; in this assay we used formaldehyde, ethanol, isopropanol, chlorine, and a commercial preparation containing 2-bromo-2-nitro-1,3-propanediol. Chlorine and the commercial preparation showed bactericidal activity (53.5% and 98.2%, respectively) for a week after disinfection.

* Corresponding author. Mailing address: Department of Preventive Medicine and Public Health, Facultad de Medicina, Universidad de Málaga, Avda Louis Pasteur, Campus Teatinos, E-29071 Málaga, Spain. Phone: 34-952131515. Fax: 34-952137131. E-mail: mariscal{at}uma.es.

Applied and Environmental Microbiology, August 1999, p. 3717-3720, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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Copyright © 1999 by the American Society for Microbiology. All rights reserved.