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Applied and Environmental Microbiology, August 1999, p. 3738-3741, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Ruminococcus flavefaciens as the Predominant Cellulolytic Bacterial Species of the Equine Cecum

Veronique Julliand,1,* Albane de Vaux,1 Liliane Millet,2 and Gerard Fonty2

Laboratoire associé de Recherches Zootechniques INRA-ENESAD, 21036 Dijon Cedex,1 and Laboratoire de Microbiologie, INRA, Centre de Recherches de Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle,2 France

Received 8 February 1999/Accepted 10 May 1999

Detection and quantification of cellulolytic bacteria with oligonucleotide probes showed that Ruminococcus flavefaciens was the predominant species in the pony and donkey cecum. Fibrobacter succinogenes and Ruminococcus albus were present at low levels. Four isolates, morphologically resembling R. flavefaciens, differed from ruminal strains by their carbohydrate utilization and their end products of cellobiose fermentation.


* Corresponding author. Mailing address: Laboratoire associé de Recherches Zootechniques INRA-ENESAD, BP 1607, 21036 Dijon Cedex, France. Phone: 33.(0)3.80.77.25.59. Fax: 33.(0)3.80.77.25.84. E-mail: v.julliand{at}enesad.fr.


Applied and Environmental Microbiology, August 1999, p. 3738-3741, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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