AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wahlström, G.
Right arrow Articles by Saris, P. E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wahlström, G.
Right arrow Articles by Saris, P. E. J.
Agricola
Right arrow Articles by Wahlström, G.
Right arrow Articles by Saris, P. E. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 1999, p. 3742-3745, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Nisin Bioassay Based on Bioluminescence

G. Wahlström1 and P. E. J. Saris2,*

Institute of Biotechnology1 and Applied Chemistry and Microbiology,2 FIN-00014 University of Helsinki, Finland

Received 29 September 1998/Accepted 6 May 1999

A Lactococcus lactis subsp. lactis strain that can sense the bacteriocin nisin and transduce the signal into bioluminescence was constructed. By using this strain, a bioassay based on bioluminescence was developed for quantification of nisin, for detection of nisin in milk, and for identification of nisin-producing strains. As little as 0.0125 ng of nisin per ml was detected within 3 h by this bioluminescence assay. This detection limit was lower than in previously described methods.

* Corresponding author. Mailing address: Department of Applied Chemistry and Microbiology, Division of Microbiology, Viikki Food Science, Viikki Biocentre, P.O. Box 56, FIN-00014 University of Helsinki, Finland. Phone: 358 9 70859369. Fax: 358 9 70859322. E-mail: per.saris{at}helsinki.fi.

Applied and Environmental Microbiology, August 1999, p. 3742-3745, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.