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Applied and Environmental Microbiology, September 1999, p. 3862-3866, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Generalized Transduction of Small Yersinia enterocolitica Plasmids

Stefan Hertwig,1,* Andreas Popp,1,dagger Barbara Freytag,1 Rudi Lurz,2 and Bernd Appel1

Department of Biological Safety, Robert Koch-Institut, D-13353 Berlin,1 and Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin,2 Germany

Received 5 April 1999/Accepted 21 June 1999

To study phage-mediated gene transfer in Yersinia, the ability of Yersinia phages to transduce naturally occurring plasmids was investigated. The transduction experiments were performed with a temperate phage isolated from a pathogenic Yersinia enterocolitica strain and phage mixtures isolated from sewage. Small plasmids (4.3 and 5.8 kb) were transduced at a frequency of 10-5 to 10-7/PFU. However, we could not detect the transduction of any indigenous virulence plasmid (ca. 72 kb) in pathogenic Yersinia strains. Transductants obtained by infection with the temperate phage were lysogenic and harbored the phage genome in their chromosomes.

* Corresponding author. Mailing address: Robert Koch-Institut, Projektgruppe 1, Nordufer 20, D-13353 Berlin, Germany. Phone: 49-30-45472113. Fax: 49-30-45472110. E-mail: Hertwigs{at}rki.de.

dagger Present address: Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, D-72076 Tübingen, Germany.

Applied and Environmental Microbiology, September 1999, p. 3862-3866, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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