This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ravenschlag, K.
Right arrow Articles by Amann, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ravenschlag, K.
Right arrow Articles by Amann, R.
Agricola
Right arrow Articles by Ravenschlag, K.
Right arrow Articles by Amann, R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 1999, p. 3982-3989, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

High Bacterial Diversity in Permanently Cold Marine Sediments

Katrin Ravenschlag, Kerstin Sahm,* Jakob Pernthaler, and Rudolf Amann

Molecular Ecology Group, Max-Planck-Institute for Marine Microbiology, D-28359 Bremen, Germany

Received 2 April 1999/Accepted 2 July 1999

A 16S ribosomal DNA (rDNA) clone library from permanently cold marine sediments was established. Screening 353 clones by dot blot hybridization with group-specific oligonucleotide probes suggested a predominance of sequences related to bacteria of the sulfur cycle (43.4% potential sulfate reducers). Within this fraction, the major cluster (19.0%) was affiliated with Desulfotalea sp. and other closely related psychrophilic sulfate reducers isolated from the same habitat. The cloned sequences showed between 93 and 100% similarity to these bacteria. Two additional groups were frequently encountered: 13% of the clones were related to Desulfuromonas palmitatis, and a second group was affiliated with Myxobacteria spp. and Bdellovibrio spp. Many clones (18.1%) belonged to the gamma  subclass of the class Proteobacteria and were closest to symbiotic or free-living sulfur oxidizers. Probe target groups were further characterized by amplified rDNA restriction analysis to determine diversity within the groups and within the clone library. Rarefaction analysis suggested that the total diversity assessed by 16S rDNA analysis was very high in these permanently cold sediments and was only partially revealed by screening of 353 clones.

* Corresponding author. Present address: Biotechnology I, Technical Microbiology, Technical University Hamburg-Harburg, Denickestr. 15, D-21073 Hamburg, Germany. Phone: 49 (0)40 42878 3336. Fax: 49 (0)40 42878 2909. E-mail: ksahm{at}mpi-bremen.de.

Applied and Environmental Microbiology, September 1999, p. 3982-3989, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Boomer, S. M., Noll, K. L., Geesey, G. G., Dutton, B. E. (2009). Formation of Multilayered Photosynthetic Biofilms in an Alkaline Thermal Spring in Yellowstone National Park, Wyoming. Appl. Environ. Microbiol. 75: 2464-2475 [Abstract] [Full Text]  
  • Harrison, B. K., Zhang, H., Berelson, W., Orphan, V. J. (2009). Variations in Archaeal and Bacterial Diversity Associated with the Sulfate-Methane Transition Zone in Continental Margin Sediments (Santa Barbara Basin, California). Appl. Environ. Microbiol. 75: 1487-1499 [Abstract] [Full Text]  
  • Pham, V. H., Yong, J.-J., Park, S.-J., Yoon, D.-N., Chung, W.-H., Rhee, S.-K. (2008). Molecular analysis of the diversity of the sulfide : quinone reductase (sqr) gene in sediment environments. Microbiology 154: 3112-3121 [Abstract] [Full Text]  
  • Dimitriu, P. A., Pinkart, H. C., Peyton, B. M., Mormile, M. R. (2008). Spatial and Temporal Patterns in the Microbial Diversity of a Meromictic Soda Lake in Washington State. Appl. Environ. Microbiol. 74: 4877-4888 [Abstract] [Full Text]  
  • Hansel, C. M., Fendorf, S., Jardine, P. M., Francis, C. A. (2008). Changes in Bacterial and Archaeal Community Structure and Functional Diversity along a Geochemically Variable Soil Profile. Appl. Environ. Microbiol. 74: 1620-1633 [Abstract] [Full Text]  
  • Qvit-Raz, N., Jurkevitch, E., Belkin, S. (2008). Drop-Size Soda Lakes: Transient Microbial Habitats on a Salt-Secreting Desert Tree. Genetics 178: 1615-1622 [Abstract] [Full Text]  
  • Kan, J., Suzuki, M. T., Wang, K., Evans, S. E., Chen, F. (2007). High Temporal but Low Spatial Heterogeneity of Bacterioplankton in the Chesapeake Bay. Appl. Environ. Microbiol. 73: 6776-6789 [Abstract] [Full Text]  
  • Elshahed, M. S., Youssef, N. H., Luo, Q., Najar, F. Z., Roe, B. A., Sisk, T. M., Buhring, S. I., Hinrichs, K.-U., Krumholz, L. R. (2007). Phylogenetic and Metabolic Diversity of Planctomycetes from Anaerobic, Sulfide- and Sulfur-Rich Zodletone Spring, Oklahoma. Appl. Environ. Microbiol. 73: 4707-4716 [Abstract] [Full Text]  
  • Mikucki, J. A., Priscu, J. C. (2007). Bacterial Diversity Associated with Blood Falls, a Subglacial Outflow from the Taylor Glacier, Antarctica. Appl. Environ. Microbiol. 73: 4029-4039 [Abstract] [Full Text]  
  • Losekann, T., Knittel, K., Nadalig, T., Fuchs, B., Niemann, H., Boetius, A., Amann, R. (2007). Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea. Appl. Environ. Microbiol. 73: 3348-3362 [Abstract] [Full Text]  
  • Holmes, D. E., Nevin, K. P., Woodard, T. L., Peacock, A. D., Lovley, D. R. (2007). Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell. Int. J. Syst. Evol. Microbiol. 57: 701-707 [Abstract] [Full Text]  
  • Perreault, N. N., Andersen, D. T., Pollard, W. H., Greer, C. W., Whyte, L. G. (2007). Characterization of the Prokaryotic Diversity in Cold Saline Perennial Springs of the Canadian High Arctic. Appl. Environ. Microbiol. 73: 1532-1543 [Abstract] [Full Text]  
  • Weidler, G. W., Dornmayr-Pfaffenhuemer, M., Gerbl, F. W., Heinen, W., Stan-Lotter, H. (2007). Communities of Archaea and Bacteria in a Subsurface Radioactive Thermal Spring in the Austrian Central Alps, and Evidence of Ammonia-Oxidizing Crenarchaeota. Appl. Environ. Microbiol. 73: 259-270 [Abstract] [Full Text]  
  • Kendall, M. M., Wardlaw, G. D., Tang, C. F., Bonin, A. S., Liu, Y., Valentine, D. L. (2007). Diversity of Archaea in Marine Sediments from Skan Bay, Alaska, Including Cultivated Methanogens, and Description of Methanogenium boonei sp. nov.. Appl. Environ. Microbiol. 73: 407-414 [Abstract] [Full Text]  
  • Lloyd, K. G., Lapham, L., Teske, A. (2006). An Anaerobic Methane-Oxidizing Community of ANME-1b Archaea in Hypersaline Gulf of Mexico Sediments. Appl. Environ. Microbiol. 72: 7218-7230 [Abstract] [Full Text]  
  • Sogin, M. L., Morrison, H. G., Huber, J. A., Welch, D. M., Huse, S. M., Neal, P. R., Arrieta, J. M., Herndl, G. J. (2006). Microbial diversity in the deep sea and the underexplored "rare biosphere". Proc. Natl. Acad. Sci. USA 103: 12115-12120 [Abstract] [Full Text]  
  • MacGregor, B. J., Boschker, H. T. S., Amann, R. (2006). Comparison of rRNA and Polar-Lipid-Derived Fatty Acid Biomarkers for Assessment of 13C-Substrate Incorporation by Microorganisms in Marine Sediments. Appl. Environ. Microbiol. 72: 5246-5253 [Abstract] [Full Text]  
  • Sattley, W. M., Madigan, M. T. (2006). Isolation, Characterization, and Ecology of Cold-Active, Chemolithotrophic, Sulfur-Oxidizing Bacteria from Perennially Ice-Covered Lake Fryxell, Antarctica. Appl. Environ. Microbiol. 72: 5562-5568 [Abstract] [Full Text]  
  • Vandieken, V., Mussmann, M., Niemann, H., Jorgensen, B. B. (2006). Desulfuromonas svalbardensis sp. nov. and Desulfuromusa ferrireducens sp. nov., psychrophilic, Fe(III)-reducing bacteria isolated from Arctic sediments, Svalbard. Int. J. Syst. Evol. Microbiol. 56: 1133-1139 [Abstract] [Full Text]  
  • Lozupone, C., Knight, R. (2005). UniFrac: a New Phylogenetic Method for Comparing Microbial Communities. Appl. Environ. Microbiol. 71: 8228-8235 [Abstract] [Full Text]  
  • Blackwood, C. B., Oaks, A., Buyer, J. S. (2005). Phylum- and Class-Specific PCR Primers for General Microbial Community Analysis. Appl. Environ. Microbiol. 71: 6193-6198 [Abstract] [Full Text]  
  • Asami, H., Aida, M., Watanabe, K. (2005). Accelerated Sulfur Cycle in Coastal Marine Sediment beneath Areas of Intensive Shellfish Aquaculture. Appl. Environ. Microbiol. 71: 2925-2933 [Abstract] [Full Text]  
  • Zhang, Y.-q., Li, Y.-z., Wang, B., Wu, Z.-h., Zhang, C.-y., Gong, X., Qiu, Z.-j., Zhang, Y. (2005). Characteristics and Living Patterns of Marine Myxobacterial Isolates. Appl. Environ. Microbiol. 71: 3331-3336 [Abstract] [Full Text]  
  • Knittel, K., Kuever, J., Meyerdierks, A., Meinke, R., Amann, R., Brinkhoff, T. (2005). Thiomicrospira arctica sp. nov. and Thiomicrospira psychrophila sp. nov., psychrophilic, obligately chemolithoautotrophic, sulfur-oxidizing bacteria isolated from marine Arctic sediments. Int. J. Syst. Evol. Microbiol. 55: 781-786 [Abstract] [Full Text]  
  • Gillan, D. C., Danis, B., Pernet, P., Joly, G., Dubois, P. (2005). Structure of Sediment-Associated Microbial Communities along a Heavy-Metal Contamination Gradient in the Marine Environment. Appl. Environ. Microbiol. 71: 679-690 [Abstract] [Full Text]  
  • Knittel, K., Losekann, T., Boetius, A., Kort, R., Amann, R. (2005). Diversity and Distribution of Methanotrophic Archaea at Cold Seeps. Appl. Environ. Microbiol. 71: 467-479 [Abstract] [Full Text]  
  • Magarvey, N. A., Keller, J. M., Bernan, V., Dworkin, M., Sherman, D. H. (2004). Isolation and Characterization of Novel Marine-Derived Actinomycete Taxa Rich in Bioactive Metabolites. Appl. Environ. Microbiol. 70: 7520-7529 [Abstract] [Full Text]  
  • Nielsen, J. L., Schramm, A., Bernhard, A. E., van den Engh, G. J., Stahl, D. A. (2004). Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries. Appl. Environ. Microbiol. 70: 7550-7554 [Abstract] [Full Text]  
  • Glavin, D. P., Cleaves, H. J., Schubert, M., Aubrey, A., Bada, J. L. (2004). New Method for Estimating Bacterial Cell Abundances in Natural Samples by Use of Sublimation. Appl. Environ. Microbiol. 70: 5923-5928 [Abstract] [Full Text]  
  • Beck, S., Schwudke, D., Strauch, E., Appel, B., Linscheid, M. (2004). Bdellovibrio bacteriovorus Strains Produce a Novel Major Outer Membrane Protein during Predacious Growth in the Periplasm of Prey Bacteria. J. Bacteriol. 186: 2766-2773 [Abstract] [Full Text]  
  • Schwudke, D., Linscheid, M., Strauch, E., Appel, B., Zahringer, U., Moll, H., Muller, M., Brecker, L., Gronow, S., Lindner, B. (2003). The Obligate Predatory Bdellovibrio bacteriovorus Possesses a Neutral Lipid A Containing {alpha}-D-Mannoses That Replace Phosphate Residues: SIMILARITIES AND DIFFERENCES BETWEEN THE LIPID As AND THE LIPOPOLYSACCHARIDES OF THE WILD TYPE STRAIN B. BACTERIOVORUS HD100 AND ITS HOST-INDEPENDENT DERIVATIVE HI100. J. Biol. Chem. 278: 27502-27512 [Abstract] [Full Text]  
  • Purdy, K. J., Nedwell, D. B., Embley, T. M. (2003). Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments. Appl. Environ. Microbiol. 69: 3181-3191 [Abstract] [Full Text]  
  • Bowman, J. P., McCammon, S. A., Gibson, J. A. E., Robertson, L., Nichols, P. D. (2003). Prokaryotic Metabolic Activity and Community Structure in Antarctic Continental Shelf Sediments. Appl. Environ. Microbiol. 69: 2448-2462 [Abstract] [Full Text]  
  • Bowman, J. P., McCuaig, R. D. (2003). Biodiversity, Community Structural Shifts, and Biogeography of Prokaryotes within Antarctic Continental Shelf Sediment. Appl. Environ. Microbiol. 69: 2463-2483 [Abstract] [Full Text]  
  • Stoeck, T., Epstein, S. (2003). Novel Eukaryotic Lineages Inferred from Small-Subunit rRNA Analyses of Oxygen-Depleted Marine Environments. Appl. Environ. Microbiol. 69: 2657-2663 [Abstract] [Full Text]  
  • Groudieva, T., Grote, R., Antranikian, G. (2003). Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen. Int. J. Syst. Evol. Microbiol. 53: 539-545 [Abstract] [Full Text]  
  • Kniemeyer, O., Fischer, T., Wilkes, H., Glockner, F. O., Widdel, F. (2003). Anaerobic Degradation of Ethylbenzene by a New Type of Marine Sulfate-Reducing Bacterium. Appl. Environ. Microbiol. 69: 760-768 [Abstract] [Full Text]  
  • Morris, C. E., Bardin, M., Berge, O., Frey-Klett, P., Fromin, N., Girardin, H., Guinebretiere, M.-H., Lebaron, P., Thiery, J. M., Troussellier, M. (2002). Microbial Biodiversity: Approaches to Experimental Design and Hypothesis Testing in Primary Scientific Literature from 1975 to 1999. Microbiol. Mol. Biol. Rev. 66: 592-616 [Abstract] [Full Text]  
  • Zengler, K., Toledo, G., Rappe, M., Elkins, J., Mathur, E. J., Short, J. M., Keller, M. (2002). Cultivating the uncultured. Proc. Natl. Acad. Sci. USA 99: 15681-15686 [Abstract] [Full Text]  
  • Hentschel, U., Hopke, J., Horn, M., Friedrich, A. B., Wagner, M., Hacker, J., Moore, B. S. (2002). Molecular Evidence for a Uniform Microbial Community in Sponges from Different Oceans. Appl. Environ. Microbiol. 68: 4431-4440 [Abstract] [Full Text]  
  • Ashelford, K. E., Weightman, A. J., Fry, J. C. (2002). PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database. Nucleic Acids Res 30: 3481-3489 [Abstract] [Full Text]  
  • Dunbar, J., Barns, S. M., Ticknor, L. O., Kuske, C. R. (2002). Empirical and Theoretical Bacterial Diversity in Four Arizona Soils. Appl. Environ. Microbiol. 68: 3035-3045 [Abstract] [Full Text]  
  • Bano, N., Hollibaugh, J. T. (2002). Phylogenetic Composition of Bacterioplankton Assemblages from the Arctic Ocean. Appl. Environ. Microbiol. 68: 505-518 [Abstract] [Full Text]  
  • Sanford, R. A., Cole, J. R., Tiedje, J. M. (2002). Characterization and Description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an Aryl-Halorespiring Facultative Anaerobic Myxobacterium. Appl. Environ. Microbiol. 68: 893-900 [Abstract] [Full Text]  
  • Joulian, C., Ramsing, N. B., Ingvorsen, K. (2001). Congruent Phylogenies of Most Common Small-Subunit rRNA and Dissimilatory Sulfite Reductase Gene Sequences Retrieved from Estuarine Sediments. Appl. Environ. Microbiol. 67: 3314-3318 [Abstract] [Full Text]  
  • Madrid, V. M., Taylor, G. T., Scranton, M. I., Chistoserdov, A. Y. (2001). Phylogenetic Diversity of Bacterial and Archaeal Communities in the Anoxic Zone of the Cariaco Basin. Appl. Environ. Microbiol. 67: 1663-1674 [Abstract] [Full Text]  
  • Orphan, V. J., Hinrichs, K.-U., Ussler, W. III, Paull, C. K., Taylor, L. T., Sylva, S. P., Hayes, J. M., Delong, E. F. (2001). Comparative Analysis of Methane-Oxidizing Archaea and Sulfate-Reducing Bacteria in Anoxic Marine Sediments. Appl. Environ. Microbiol. 67: 1922-1934 [Abstract] [Full Text]  
  • Gieseke, A., Purkhold, U., Wagner, M., Amann, R., Schramm, A. (2001). Community Structure and Activity Dynamics of Nitrifying Bacteria in a Phosphate-Removing Biofilm. Appl. Environ. Microbiol. 67: 1351-1362 [Abstract] [Full Text]  
  • Detmers, J., Brüchert, V., Habicht, K. S., Kuever, J. (2001). Diversity of Sulfur Isotope Fractionations by Sulfate-Reducing Prokaryotes. Appl. Environ. Microbiol. 67: 888-894 [Abstract] [Full Text]  
  • Ravenschlag, K., Sahm, K., Amann, R. (2001). Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard). Appl. Environ. Microbiol. 67: 387-395 [Abstract] [Full Text]  
  • Barbieri, E., Potenza, L., Rossi, I., Sisti, D., Giomaro, G., Rossetti, S., Beimfohr, C., Stocchi, V. (2000). Phylogenetic Characterization and In Situ Detection of a Cytophaga-Flexibacter-Bacteroides Phylogroup Bacterium in Tuber borchii Vittad. Ectomycorrhizal Mycelium. Appl. Environ. Microbiol. 66: 5035-5042 [Abstract] [Full Text]  
  • Bull, A. T., Ward, A. C., Goodfellow, M. (2000). Search and Discovery Strategies for Biotechnology: the Paradigm Shift. Microbiol. Mol. Biol. Rev. 64: 573-606 [Abstract] [Full Text]  
  • Ravenschlag, K., Sahm, K., Knoblauch, C., Jørgensen, B. B., Amann, R. (2000). Community Structure, Cellular rRNA Content, and Activity of Sulfate-Reducing Bacteria in Marine Arctic Sediments. Appl. Environ. Microbiol. 66: 3592-3602 [Abstract] [Full Text]  
  • Thamdrup, B., Rosselló-Mora, R., Amann, R. (2000). Microbial Manganese and Sulfate Reduction in Black Sea Shelf Sediments. Appl. Environ. Microbiol. 66: 2888-2897 [Abstract] [Full Text]  
  • Eilers, H., Pernthaler, J., Glöckner, F. O., Amann, R. (2000). Culturability and In Situ Abundance of Pelagic Bacteria from the North Sea. Appl. Environ. Microbiol. 66: 3044-3051 [Abstract] [Full Text]  
  • Sahm, K., Knoblauch, C., Amann, R. (1999). Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-Reducing Isolates in Marine Arctic Sediments. Appl. Environ. Microbiol. 65: 3976-3981 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»