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Applied and Environmental Microbiology, September 1999, p. 4071-4076, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Standardization of Mating Type Terminology in the Gibberella fujikuroi Species Complexdagger

Z. Kerényi,1 K. Zeller,2 L. Hornok,1 and J. F. Leslie2,*

Agricultural Biotechnology Center, Gödöllo, Hungary,1 and Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506-55022

Received 8 March 1999/Accepted 15 June 1999

Mating type in the Gibberella fujikuroi species complex is controlled by a single locus with two alleles and is usually identified following sexual crosses with standard, female-fertile tester isolates. The mating type alleles have been arbitrarily designated "+" and "-" within each biological species, and the nomenclature is tied to the standard tester strains. We developed a pair of PCR primers that can be used to amplify a unique fragment of one of the mating type alleles (MAT-2) from at least seven of the biological species in this species complex. Based on the amplification pattern, we propose a replacement for the existing, arbitrary +/- terminology that is presently in use. The new terminology is based on DNA sequence similarities between the mating type allele fragments from the biological species of the G. fujikuroi species complex and the corresponding fragments from other filamentous ascomycetes.

* Corresponding author. Mailing address: Department of Plant Pathology, 4002 Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502. Phone: (785) 532-1363. Fax: (785) 532-2414. E-mail: jfl{at}plantpath.ksu.edu.

dagger Contribution no. 99-376-J from the Kansas Agricultural Experiment Station, Manhattan.

Applied and Environmental Microbiology, September 1999, p. 4071-4076, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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