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Applied and Environmental Microbiology, September 1999, p. 4223-4226, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation of Nitrogen-Fixing Bacteria Containing Molybdenum-Independent Nitrogenases from Natural Environments

Telisa M. Loveless, J. Royden Saah, and Paul E. Bishop*

USDA Agricultural Research Service and Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695-7615

Received 13 January 1999/Accepted 8 June 1999

Seven diazotrophs that grow well under Mo-deficient, N2-fixing conditions were isolated from a variety of environments. These isolates fall in the gamma  subdivision of the class Proteobacteria and have genes that encode the Mo nitrogenase (nitrogenase 1) and the V nitrogenase (nitrogenase 2). Four of the isolates also harbor genes that encode the iron-only nitrogenase (nitrogenase 3).

* Corresponding author. Mailing address: Department of Microbiology, North Carolina State University, Raleigh, NC 27695-7615. Phone: (919) 515-3770. Fax: (919) 515-7867. E-mail: peb{at}mbio.mbio.ncsu.edu.

Applied and Environmental Microbiology, September 1999, p. 4223-4226, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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