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Applied and Environmental Microbiology, September 1999, p. 4245-4257, Vol. 65, No. 9
Unité des Entérobactéries,
Unité INSERM 389, Institut Pasteur, 75724 Paris Cedex 15, France
Received 16 February 1999/Accepted 17 June 1999
The influence of growth temperature on the ability to ferment
D-sorbitol was investigated in Escherichia coli
O157:H7. It was found that O157:H7 strains have a temperature-sensitive
sorbitol phenotype. D-Sorbitol transport and
sorbitol-6-phosphate dehydrogenase activities were expressed in
sorbitol-fermenting cells grown at 30°C but only at a low level at
40°C. Sorbitol-positive variants able to transport
D-sorbitol were easily selected at 30°C from culture of
Sor
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Temperature-Dependent Fermentation of
D-Sorbitol in Escherichia coli O157:H7
E. coli O157:H7 strains.
*
Corresponding author. Mailing address: Unité des
Entérobactéries, Institut Pasteur, 75724 Paris Cedex 15, France. Phone: 33-1-40-61-36-73. Fax: 33-1-45-68-88-37. E-mail:
obouvet{at}pasteur.fr.
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