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Applied and Environmental Microbiology, September 1999, p. 4255-4260, Vol. 65, No. 9
0099-2240/99/$04.00+0

Bacterial Spores Survive Treatment with Commercial Sterilants and Disinfectants

Jose-Luis Sagripanti* and Aylin Bonifacino

Molecular Biology Branch (HFZ-113), Division of Life Sciences, Office of Science and Technology, Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland

Received 22 February 1999/Accepted 9 June 1999

This study compared the activity of commercial liquid sterilants and disinfectants on Bacillus subtilis spores deposited on three types of devices made of noncorrodible, corrodible, or polymeric material. Products like Renalin, Exspor, Wavicide-01, Cidexplus, and cupric ascorbate were tested under conditions specified for liquid sterilization. These products, at the shorter times indicated for disinfection, and popular disinfectants, like Clorox, Cavicide, and Lysol were also studied. Data obtained with a sensitive and quantitative test suggest that commercial liquid sterilants and disinfectants are less effective on contaminated surfaces than generally acknowledged.

* Corresponding author. Mailing address: Molecular Biology Branch (HFZ-113), Center for Devices and Radiological Health, Food and Drug Administration, 5600 Fishers Ln., Rockville, MD 20857. E-mail: JUS{at}CDRH.FDA.GOV.

Applied and Environmental Microbiology, September 1999, p. 4255-4260, Vol. 65, No. 9
0099-2240/99/$04.00+0


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