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Applied and Environmental Microbiology, September 1999, p. 4261-4263, Vol. 65, No. 9
0099-2240/99/$04.00+0

Perkinsus marinus Extracellular Protease Modulates Survival of Vibrio vulnificus in Eastern Oyster (Crassostrea virginica) Hemocytesdagger

B. D. Tall,1,* J. F. La Peyre,2,Dagger J. W. Bier,3 M. D. Miliotis,4 D. E. Hanes,4 M. H. Kothary,4 D. B. Shah,1 and M. Faisal2

Divisions of Microbiological Studies,1 Science and Applied Technology,3 and Virulence Assessment,4 Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204 and School of Marine Science, Virginia Institute of Marine Science, The College of William and Mary, Gloucester Point, Virginia 230622

Received 24 May 1999/Accepted 1 July 1999

The in vitro effects of the Perkinsus marinus serine protease on the intracellular survival of Vibrio vulnificus in oyster hemocytes were examined by using a time-course gentamicin internalization assay. Results showed that protease-treated hemocytes were initially slower to internalize V. vulnificus than untreated hemocytes. After 1 h, the elimination of V. vulnificus by treated hemocytes was significantly suppressed compared with hemocytes infected with invasive and noninvasive controls. Our data suggest that the serine protease produced by P. marinus suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in oyster tissues.

* Corresponding author. Mailing address: Division of Microbiological Studies (HFS-517), Food and Drug Administration, 200 C St. S.W., Washington, D.C. 20204. Phone: (202) 205-4648. Fax: (202) 401-7740. E-mail: BTall{at}bangate.fda.gov.

dagger Virginia Institute of Marine Science contribution number 2240.

Dagger Present address: Department of Veterinary Science, Louisiana State University, Baton Rouge, LA 70803.

Applied and Environmental Microbiology, September 1999, p. 4261-4263, Vol. 65, No. 9
0099-2240/99/$04.00+0


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