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Applied and Environmental Microbiology, September 1999, p. 4280-4284, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Verrucomicrobia in a Pasture Soil by PCR-Mediated Amplification of 16S rRNA Genes

Katrina A. O'Farrell and Peter H. Janssen*

Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052, Australia

Received 11 March 1999/Accepted 13 July 1999

Oligonucleotide primers were designed and used to amplify, by PCR, partial 16S rRNA genes of members of the bacterial division Verrucomicrobia in DNA extracted from a pasture soil. By applying most-probable-number theory to the assay, verrucomicrobia appeared to contribute some 0.2% of the soil DNA. Amplified ribosomal DNA restriction analysis of 53 cloned PCR-amplified partial 16S rRNA gene fragments and comparative sequence analysis of 21 nonchimeric partial 16S rRNA genes showed that these primers amplified only 16S rRNA genes of members of the Verrucomicrobia in DNA extracted from the soil.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052, Australia. Phone: 61 (3) 9344 5706. Fax: 61 (3) 9347 1540. E-mail: p.janssen{at}microbiology.unimelb.edu.au.

Applied and Environmental Microbiology, September 1999, p. 4280-4284, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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