This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wuytack, E. Y.
Right arrow Articles by Michiels, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wuytack, E. Y.
Right arrow Articles by Michiels, C. W.
Agricola
Right arrow Articles by Wuytack, E. Y.
Right arrow Articles by Michiels, C. W.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2000, p. 257-261, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparative Study of Pressure- and Nutrient-Induced Germination of Bacillus subtilis Spores

Elke Y. Wuytack, Johan Soons, Filip Poschet, and Chris W. Michiels*

Laboratory of Food Microbiology, Katholieke Universiteit Leuven, Leuven, Belgium

Received 6 July 1999/Accepted 22 October 1999

Germination experiments with specific germination mutants of Bacillus subtilis, including a newly isolated mutant affected in pressure-induced germination, suggest that a pressure of 100 MPa triggers the germination cascades that are induced by the nutrient germinant alanine (Ala) and by a mixture of asparagine, glucose, fructose, and potassium ions (AGFK), by activating the receptors for alanine and asparagine, GerA and GerB, respectively. As opposed to germination at 100 MPa, germination at 600 MPa apparently shortcuts at least part of the Ala- and AGFK-induced germination pathways. Inhibitors of nutrient-induced germination (HgCl2 and Nalpha -P-tosyl-L-arginine methyl ester) also inhibit pressure-induced germination at 600 MPa, suggesting that germination at 600 MPa involves activation of a true physiological germination pathway and is therefore not merely a physico-chemical process in which water is forced into the spore protoplast.

* Corresponding author. Mailing address: Laboratory of Food Microbiology, Kard. Mercierlaan 92, B-3001 Heverlee, Belgium. Phone: 32-16-32.15.78. Fax: 32-16-32.19.60. E-mail: chris.michiels{at}agr.kuleuven.ac.be.

Applied and Environmental Microbiology, January 2000, p. 257-261, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pelczar, P. L., Igarashi, T., Setlow, B., Setlow, P. (2007). Role of GerD in Germination of Bacillus subtilis Spores. J. Bacteriol. 189: 1090-1098 [Abstract] [Full Text]  
  • Cote, C. K., Van Rooijen, N., Welkos, S. L. (2006). Roles of Macrophages and Neutrophils in the Early Host Response to Bacillus anthracis Spores in a Mouse Model of Infection. Infect. Immun. 74: 469-480 [Abstract] [Full Text]  
  • Black, E. P., Koziol-Dube, K., Guan, D., Wei, J., Setlow, B., Cortezzo, D. E., Hoover, D. G., Setlow, P. (2005). Factors Influencing Germination of Bacillus subtilis Spores via Activation of Nutrient Receptors by High Pressure. Appl. Environ. Microbiol. 71: 5879-5887 [Abstract] [Full Text]  
  • Hornstra, L. M., de Vries, Y. P., de Vos, W. M., Abee, T., Wells-Bennik, M. H. J. (2005). gerR, a Novel ger Operon Involved in L-Alanine- and Inosine-Initiated Germination of Bacillus cereus ATCC 14579. Appl. Environ. Microbiol. 71: 774-781 [Abstract] [Full Text]  
  • Margosch, D., Ganzle, M. G., Ehrmann, M. A., Vogel, R. F. (2004). Pressure Inactivation of Bacillus Endospores. Appl. Environ. Microbiol. 70: 7321-7328 [Abstract] [Full Text]  
  • de Vries, Y. P., Hornstra, L. M., de Vos, W. M., Abee, T. (2004). Growth and Sporulation of Bacillus cereus ATCC 14579 under Defined Conditions: Temporal Expression of Genes for Key Sigma Factors. Appl. Environ. Microbiol. 70: 2514-2519 [Abstract] [Full Text]  
  • Clery-Barraud, C., Gaubert, A., Masson, P., Vidal, D. (2004). Combined Effects of High Hydrostatic Pressure and Temperature for Inactivation of Bacillus anthracis Spores. Appl. Environ. Microbiol. 70: 635-637 [Abstract] [Full Text]  
  • Dogan, C., Erkmen, O. (2003). Note: Ultra High Hydrostatic Pressure Inactivation of Escherichia Coli in Milk, and Orange and Peach Juices. Food Science and Technology International 9: 403-407 [Abstract]  
  • Barlass, P. J., Houston, C. W., Clements, M. O., Moir, A. (2002). Germination of Bacillus cereus spores in response to L-alanine and to inosine: the roles of gerL and gerQ operons. Microbiology 148: 2089-2095 [Abstract] [Full Text]  
  • Paidhungat, M., Setlow, B., Daniels, W. B., Hoover, D., Papafragkou, E., Setlow, P. (2002). Mechanisms of Induction of Germination of Bacillus subtilis Spores by High Pressure. Appl. Environ. Microbiol. 68: 3172-3175 [Abstract] [Full Text]  
  • Nicholson, W. L., Munakata, N., Horneck, G., Melosh, H. J., Setlow, P. (2000). Resistance of Bacillus Endospores to Extreme Terrestrial and Extraterrestrial Environments. Microbiol. Mol. Biol. Rev. 64: 548-572 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»