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Applied and Environmental Microbiology, January 2000, p. 359-362, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Requirement of Monooxygenase-Mediated Steps for Sterigmatocystin Biosynthesis by Aspergillus nidulans

Nancy P. Keller,1,* Coran M. H. Watanabe,2 Hemant S. Kelkar,1,3,dagger Thomas H. Adams,3,Dagger and Craig A. Townsend2

Department of Plant Pathology and Microbiology1 and Department of Biology,3 Texas A&M University, College Station, Texas, and Department of Chemistry, Johns Hopkins University, Baltimore, Maryland2

Received 8 June 1999/Accepted 2 November 1999

Sterigmatocystin (ST) and aflatoxin B1 (AFB1) are two polyketide-derived Aspergillus mycotoxins synthesized by functionally identical sets of enzymes. ST, the compound produced by Aspergillus nidulans, is a late intermediate in the AFB1 pathway of A. parasiticus and A. flavus. Previous biochemical studies predicted that five oxygenase steps are required for the formation of ST. A 60-kb ST gene cluster in A. nidulans contains five genes, stcB, stcF, stcL, stcS, and stcW, encoding putative monooxygenase activities. Prior research showed that stcL and stcS mutants accumulated versicolorins B and A, respectively. We now show that strains disrupted at stcF, encoding a P-450 monooxygenase similar to A. parasiticus avnA, accumulate averantin. Disruption of either StcB (a putative P-450 monooxygenase) or StcW (a putative flavin-requiring monooxygenase) led to the accumulation of averufin as determined by radiolabeled feeding and extraction studies.

* Corresponding author. Mailing address: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843. Phone: (409) 845-0963. Fax: (409) 845-6483. E-mail: n-keller{at}tamu.edu.

dagger Present address: Department of Genetics, University of Georgia, Athens, GA 30602.

Dagger Present address: DeKalb Genetics, Mystic, CT 06355.

Applied and Environmental Microbiology, January 2000, p. 359-362, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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