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Applied and Environmental Microbiology, January 2000, p. 36-41, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Endo-Xylogalacturonan Hydrolase, a Novel Pectinolytic Enzyme

C. J. B. van der Vlugt-Bergmans,1,* P. J. A. Meeuwsen,2 A. G. J. Voragen,2 and A. J. J. van Ooyen1

Industrial Microbiology Group,1 and Food Chemistry Group,2 Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, NL-6700 EV Wageningen, The Netherlands

Received 1 March 1999/Accepted 1 October 1999

We screened an Aspergillus tubingensis expression library constructed in the yeast Kluyveromyces lactis for xylogalacturonan-hydrolyzing activity in microwell plates by using a bicinchoninic acid assay. This assay detects reducing carbohydrate groups when they are released from a carbohydrate by enzymatic activity. Two K. lactis recombinants exhibiting xylogalacturonan-hydrolyzing activity were found among the 3,400 colonies tested. The cDNA insert of these recombinants encoded a 406-amino-acid protein, designated XghA, which was encoded by a single-copy gene, xghA. A multiple-sequence alignment revealed that XghA was similar to both polygalacturonases (PGs) and rhamnogalacturonases. A detailed examination of conserved regions in the sequences of these enzymes revealed that XghA resembled PGs more. High-performance liquid chromatography and matrix-assisted laser desorption ionization-time of flight mass spectrometry of the products of degradation of xylogalacturonan and saponified modified hairy regions of apple pectin by XghA demonstrated that this enzyme uses an endo type of mechanism. XghA activity appeared to be specific for a xylose-substituted galacturonic acid backbone.

* Corresponding author. Mailing address: Industrial Microbiology Group, Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, P.O. Box 8129, NL-6700 EV Wageningen, The Netherlands. Phone: 31 317 484980. Fax: 31 317 484978. E-mail: cecile.vandervlugt.imb.ftns.wau.nl.

Applied and Environmental Microbiology, January 2000, p. 36-41, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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