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Applied and Environmental Microbiology, January 2000, p. 428-430, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pulsed-Electric Field Treatment Enhances the Bactericidal Action of Nisin against Bacillus cereus

Irene E. Pol,* Hennie C. Mastwijk, Paul V. Bartels, and Eddy J. Smid

Agrotechnological Research Institute (ATO-DLO), 6708 PD Wageningen, The Netherlands

Received 24 March 1999/Accepted 28 September 1999

Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 µg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-µs duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatments, indicating synergy.

* Corresponding author. Mailing address: ATO-DLO, Department of Applied Microbiology, Bornsesteeg 59, 6708 GA Wageningen, The Netherlands. Phone: 31.317.475108. Fax: 31.317.475.347. E-mail: I.E.Pol{at}ATO.DLO.NL.

Applied and Environmental Microbiology, January 2000, p. 428-430, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Somolinos, M., Garcia, D., Condon, S., Manas, P., Pagan, R. (2007). Relationship between Sublethal Injury and Inactivation of Yeast Cells by the Combination of Sorbic Acid and Pulsed Electric Fields. Appl. Environ. Microbiol. 73: 3814-3821 [Abstract] [Full Text]  
  • Pol, I. E., van Arendonk, W. G. C., Mastwijk, H. C., Krommer, J., Smid, E. J., Moezelaar, R. (2001). Sensitivities of Germinating Spores and Carvacrol-Adapted Vegetative Cells and Spores of Bacillus cereus to Nisin and Pulsed-Electric-Field Treatment. Appl. Environ. Microbiol. 67: 1693-1699 [Abstract] [Full Text]  


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