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Applied and Environmental Microbiology, January 2000, p. 439-442, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Use of Sublimation To Prepare Solid Microbial Media with Water-Insoluble Substrates

Jeremy F. Alley* and Lewis R. Brown

Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi 39762-5759

Received 16 June 1999/Accepted 17 October 1999

A method was developed to deposit a visible layer of water-insoluble compounds via sublimation onto the surface of solid media. The compound is sublimed from a heated aluminum dish containing the compound onto the surface of an inverted, ice-cooled, inoculated agar petri dish. The method results in the deposition of a thin, even layer on the agar surface without the use of solvent. After incubation, clearing zones around colonies indicate the presence of compound-degrading microorganisms.

* Corresponding author. Mailing address: Department of Biological Sciences, P.O. Drawer GY, Mississippi State University, Mississippi State, MS 39762-5759. Phone: (662) 325-7592. Fax: (662) 325-7939. E-mail: jfa2{at}ra.msstate.edu.

Applied and Environmental Microbiology, January 2000, p. 439-442, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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