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Applied and Environmental Microbiology, October 2000, p. 4200-4204, Vol. 66, No. 10
Department of Animal Science, McGill
University/Macdonald Campus, Ste Anne de
Bellevue,1 and Department of Animal
Science, University of Manitoba, Winnipeg,2
Canada
Received 3 April 2000/Accepted 28 July 2000
Few studies, if any, have addressed the adhesion of enterococci to
the intestinal mucosa and their interference with the adhesion of
pathogens, although more than 60% of probiotic preparations in the
market contain strains of enterococci. The objective of this study was
to investigate if Enterococcus faecium 18C23 has the
ability to inhibit the adhesion of Escherichia coli K88ac and K88MB to the small intestine mucus of piglets. Approximately 9% of
E. faecium 18C23 organisms adhered to the small intestine mucus, and the adhesion was found to be specific. Living E. faecium 18C23 culture efficiently inhibited the adhesion of
E. coli K88ac and K88MB to the piglet intestine mucus.
Inhibition of the adhesion of E. coli K88ac to the small
intestine mucus was found to be dose dependent. Inhibition of >90%
was observed when 109 CFU or more of living E. faecium 18C23 culture per ml was added simultaneously with
E. coli to immobilized mucus. The substances from both the
18C23 cells and the spent culture supernatant contributed to the
inhibition of adhesion of E. coli K88 to the small
intestine mucus receptors. The inhibiting effect was not solely a pH
effect since considerable inhibitory action was demonstrated after
neutralizing the mixture or spent culture supernatant to pH 7.0. Part
of the inhibition of adhesion of E. coli K88ac by E. faecium 18C23 or its supernatant might occur through steric hindrance.
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
A Strain of Enterococcus faecium (18C23)
Inhibits Adhesion of Enterotoxigenic Escherichia coli K88 to
Porcine Small Intestine Mucus
*
Corresponding author. Mailing address: Department of
Animal Science, McGill University, Macdonald Campus, 21111 Lakeshore Rd., Ste Anne de Bellevue, QC, H9X 3V9 Canada. Phone: (514) 398-7975. Fax: (514) 398-7964. E-mail: Zhao{at}macdonald.McGill.ca.
Applied and Environmental Microbiology, October 2000, p. 4200-4204, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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