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Applied and Environmental Microbiology, October 2000, p. 4408-4413, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Correlations between Morphological, Molecular Biological, and Physiological Characteristics in Clinical and Nonclinical Isolates of Acanthamoeba spp.

Julia Walochnik, Andreas Obwaller, and Horst Aspöck*

Department for Medical Parasitology, Clinical Institute of Hygiene, University of Vienna, 1095 Vienna, Austria

Received 3 May 2000/Accepted 26 July 2000

Eleven Acanthamoeba isolates, obtained from Acanthamoeba keratitis patients, from contact lens cases of non-Acanthamoeba keratitis patients, from asymptomatic individuals, from necrotic tissue, and from tap water and two reference strains were investigated by morphological, molecular biological, and physiological means in order to discriminate clinically relevant and nonrelevant isolates. All clinically relevant isolates showed Acanthamoeba sp. group II morphology. 18S ribosomal DNA sequencing revealed sequence type T4 to be the most prevalent group among the isolates and also the group recruiting most of the pathogenic strains. Interestingly, within T4 the strains of no clinical relevance clustered together. Moreover, physiological properties appeared to be highly consistent with initial pathogenicity and with sequence clustering. Altogether, the results of our study indicate a correlation between the phylogenetic relationship and pathogenicity.

* Corresponding author. Mailing address: Department for Medical Parasitology, Clinical Institute of Hygiene, University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria. Phone: 043-1-4277-79430. Fax: 043-1-4277-9794. E-mail: Horst.Aspoeck{at}univie.ac.at.

Applied and Environmental Microbiology, October 2000, p. 4408-4413, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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