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Applied and Environmental Microbiology, October 2000, p. 4555-4558, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid and Simple Determination of the Escherichia coli Phylogenetic Group

Olivier Clermont, Stéphane Bonacorsi, and Edouard Bingen*

Laboratoire d'études de génétique bactérienne dans les infections de l'enfant (EA3105), Université Denis Diderot-Paris 7, Hôpital Robert Debré, Paris, France

Received 15 February 2000/Accepted 7 July 2000

Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on triplex PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Robert Debré, 48 blvd. Sérurier, 75395 Paris cedex 19, France. Phone: 33 1 40 03 23 40. Fax: 33 1 40 03 24 50. E-mail: edouard.bingen{at}rdb.ap-hop-paris.fr.

Applied and Environmental Microbiology, October 2000, p. 4555-4558, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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