This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Magnuson, M. L.
Right arrow Articles by Kelty, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Magnuson, M. L.
Right arrow Articles by Kelty, C. A.
Agricola
Right arrow Articles by Magnuson, M. L.
Right arrow Articles by Kelty, C. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2000, p. 4720-4724, Vol. 66, No. 11
0099-2240/00/$04.00+0

Characterization of Cryptosporidium parvum by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Matthew L. Magnuson,1,* James H. Owens,2 and Catherine A. Kelty1

Treatment Technology Evaluation Branch1 and Microbial Contaminants Control Branch,2 National Risk Management Research Laboratory, Water Supply and Water Resources Division, Office of Research and Development, United States Environmental Protection Agency, Cincinnati, Ohio 45268

Received 26 June 2000/Accepted 15 August 2000

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to investigate whole and freeze-thawed Cryptosporidium parvum oocysts. Whole oocysts revealed some mass spectral features. Reproducible patterns of spectral markers and increased sensitivity were obtained after the oocysts were lysed with a freeze-thaw procedure. Spectral-marker patterns for C. parvum were distinguishable from those obtained for Cryptosporidium muris. One spectral marker appears specific for the genus, while others appear specific at the species level. Three different C. parvum lots were investigated, and similar spectral markers were observed in each. Disinfection of the oocysts reduced and/or eliminated the patterns of spectral markers.

* Corresponding author. Mailing address: U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268. Phone: (513) 569-7321. Fax: (513) 569-7658. E-mail: Magnuson.Matthew{at}epa.gov.

Applied and Environmental Microbiology, November 2000, p. 4720-4724, Vol. 66, No. 11
0099-2240/00/$04.00+0


This article has been cited by other articles:

  • Halden, R. U., Colquhoun, D. R., Wisniewski, E. S. (2005). Identification and Phenotypic Characterization of Sphingomonas wittichii Strain RW1 by Peptide Mass Fingerprinting Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Appl. Environ. Microbiol. 71: 2442-2451 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»