AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnsen, L.
Right arrow Articles by Nissen-Meyer, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnsen, L.
Right arrow Articles by Nissen-Meyer, J.
Agricola
Right arrow Articles by Johnsen, L.
Right arrow Articles by Nissen-Meyer, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2000, p. 4798-4802, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Engineering Increased Stability in the Antimicrobial Peptide Pediocin PA-1

Line Johnsen,1 Gunnar Fimland,1,* Vincent Eijsink,2 and Jon Nissen-Meyer1

Department of Biochemistry, University of Oslo, Oslo,1 and Department of Chemistry and Biotechnology, Agricultural University of Norway, Ås,2 Norway

Received 3 April 2000/Accepted 18 August 2000

Pediocin PA-1 is a food grade antimicrobial peptide that has been used as a food preservative. Upon storage at 4°C or room temperature, pediocin PA-1 looses activity, and there is a concomitant 16-Da increase in the molecular mass. It is shown that the loss of activity follows first-order kinetics and that the instability can be prevented by replacing the single methionine residue (Met31) in pediocin PA-1. Replacing Met by Ala, Ile, or Leu protected the peptide from oxidation and had only minor effects on bacteriocin activity (for most indicator strains 100% activity was maintained). Replacement of Met by Asp was highly deleterious for bacteriocin activity.

* Corresponding author. Mailing address: Department of Biochemistry, University of Oslo, Post Box 1041, Blindern, 0316 Oslo, Norway. Phone: 47-22 85 66 32 or 47-22 85 73 51. Fax: 47-22 85 44 43. E-mail: gunnar.fimland{at}biokjemi.uio.no.

Applied and Environmental Microbiology, November 2000, p. 4798-4802, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2000 by the American Society for Microbiology. All rights reserved.