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Applied and Environmental Microbiology, November 2000, p. 4834-4841, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Application of Siderotyping for Characterization of Pseudomonas tolaasii and "Pseudomonas reactans" Isolates Associated with Brown Blotch Disease of Cultivated Mushrooms

Patricia Munsch,1 Valerie A. Geoffroy,2 Tapani Alatossava,1 and Jean-Marie Meyer2,*

Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, 88600 Sotkamo, Finland,1 and Laboratoire de Microbiologie et de Génétique, CNRS UPRES-A 7010, Université Louis-Pasteur, 67000 Strasbourg, France2

Received 23 March 2000/Accepted 2 August 2000

Pyoverdine isoelectric focusing analysis and pyoverdine-mediated iron uptake were used as siderotyping methods to analyze a collection of 57 northern and central European isolates of P. tolaasii and "P. reactans." The bacteria, isolated from cultivated Agaricus bisporus or Pleurotus ostreatus mushroom sporophores presenting brown blotch disease symptoms, were identified according to the white line test (W. C. Wong and T. F. Preece, J. Appl. Bacteriol. 47:401-407, 1979) and their pathogenicity towards A. bisporus and were grouped into siderovars according to the type of pyoverdine they produced. Seventeen P. tolaasii isolates were recognized, which divided into two siderovars, with the first one containing reference strains and isolates of various geographical origins while the second one contained Finnish isolates exclusively. The 40 "P. reactans" isolates divided into eight siderovars. Pyoverdine isoelectric focusing profiles and cross-uptake studies demonstrated an identity for some "P. reactans" isolates, with reference strains belonging to the P. fluorescens biovars II, III, or V. Thus, the easy and rapid methods of siderotyping proved to be reliable by supporting and strengthening previous taxonomical data. Moreover, two potentially novel pyoverdines characterizing one P. tolaasii siderovar and one "P. reactans" siderovar were found.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et de Génétique, 28 rue Goethe, 67083 Strasbourg Cedex, France. Phone (33) 3 88 24 41 50. Fax: (33) 3 88 35 84 84. E-mail: meyer{at}gem.u-strasbg.fr.

Applied and Environmental Microbiology, November 2000, p. 4834-4841, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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