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Applied and Environmental Microbiology, November 2000, p. 4926-4934, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Persistent Colonization of Sheep by
Escherichia coli O157:H7 and Other E. coli
Pathotypes
N. A.
Cornick,1,*
S. L.
Booher,1
T. A.
Casey,2 and
H. W.
Moon1
Veterinary Medical Research Institute, Iowa
State University,1 and Enteric Diseases
and Food Safety Research Unit, National Animal Disease Center, UDSA
Agricultural Research Service,2 Ames, Iowa 50011
Received 4 February 2000/Accepted 29 August 2000
Shiga toxin-producing Escherichia coli (STEC) is an
important cause of food-borne illness in humans. Ruminants appear to be more frequently colonized by STEC than are other animals, but the
reason(s) for this is unknown. We compared the frequency, magnitude,
duration, and transmissibility of colonization of sheep by E. coli O157:H7 to that by other pathotypes of E. coli.
Young adult sheep were simultaneously inoculated with a cocktail
consisting of two strains of E. coli O157:H7, two strains
of enterotoxigenic E. coli (ETEC), and one strain of
enteropathogenic E. coli. Both STEC strains and ETEC 2041 were given at either 107 or 1010
CFU/strain/animal. The other strains were given only at
1010 CFU/strain. We found no consistent differences among
pathotypes in the frequency, magnitude, and transmissibility of
colonization. However, the STEC strains tended to persist to 2 weeks
and 2 months postinoculation more frequently than did the other
pathotypes. The tendency for persistence of the STEC strains was
apparent following an inoculation dose of either 107 or
1010 CFU. One of the ETEC strains also persisted when
inoculated at 1010 CFU. However, in contrast to the STEC
strains, it did not persist when inoculated at 107 CFU.
These results support the hypothesis that STEC is better adapted to
persist in the alimentary tracts of sheep than are other pathotypes of
E. coli.
*
Corresponding author. Mailing address: Veterinary
Medical Research Institute, 1802 Elwood Dr., Ames, IA 50011. Phone:
(515) 294-6236. Fax: (515) 294-1401. E-mail:
ncornick{at}iastate.edu.
Applied and Environmental Microbiology, November 2000, p. 4926-4934, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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