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Applied and Environmental Microbiology, November 2000, p. 5030-5034, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Colonization of the Stratified Squamous Epithelium of the Nonsecreting Area of Horse Stomach by Lactobacilli

Norikatsu Yuki,¹ Tomoko Shimazaki,^2, Akira Kushiro,¹ Koichi Watanabe,¹ Kazumi Uchida,¹ Teruhiko Yuyama,³ and Masami Morotomi^1,*

Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650,¹ School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628,² and The Japan Bloodhorse Breeder's Association Kagoshima Stallion Station, 3995 Nogata, Oosaki, Oso, Kagoshima 899-8313,³ Japan

Received 12 May 2000/Accepted 23 August 2000

Selective adhesion to only certain epithelia is particularly common among the bacterial members of the indigenous microflora of mammals. We have found that the stratified squamous epithelium of the nonsecreting area of horse stomach is colonized by gram-positive rods. The microscopic features of a dense layer of these bacteria on the epithelium were found to be similar to those reported in mice, rats, and swine. Adhering microorganisms were isolated and identified as Lactobacillus salivarius, L. crispatus, L. reuteri, and L. agilis by DNA-DNA hybridization and 16S rRNA gene sequencing techniques. These lactobacilli associated with the horse, except for L. reuteri, were found to adhere to horse epithelial cells in vitro but not to those of rats. A symbiotic relationship of these lactobacilli with the horse is suggested.

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^* Corresponding author. Mailing address: Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, Japan. Phone: 81-42-577-8960. Fax: 81-42-577-3020. E-mail: masami-morotomi{at}yakult.co.jp.

Present address: Graduate student of Keio University School of Medicine, 35 Shinanomachi, Sinjuku, Tokyo 160-8582, Japan.

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Applied and Environmental Microbiology, November 2000, p. 5030-5034, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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