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Applied and Environmental Microbiology, November 2000, p. 5083-5086, Vol. 66, No. 11
Laboratoire de Microbiologie, Faculté de
Médecine de Tours,1 and
Département de Microbiologie Médicale et
Moléculaire, Unité de Bactériologie, Centre
Hospitalier Universitaire Bretonneau,2 37032 Tours Cedex, France
Received 24 April 2000/Accepted 15 August 2000
Ninety-seven epidemiologically unrelated strains of Listeria
monocytogenes were investigated for their sensitivities to
quaternary ammonium compounds (benzalkonium chloride and cetrimide).
The MICs for seven serogroup 1/2 strains were high. Three came from the
environment and four came from food; none were isolated from human or
animal samples. All 97 strains carried the mdrL gene, which
encodes a multidrug efflux pump, and the orfA gene, a
putative transcriptional repressor of mdrL. The absence of
plasmids in four of the seven resistant strains and the conservation of
resistance after plasmid curing suggested that the resistance genes are
not plasmid borne. Moreover, PCR amplification and Southern blot
hybridization experiments failed to find genes phylogenetically related
to the qacA and smr genes, encoding multidrug
efflux systems previously described for the genus
Staphylococcus. The high association between nontypeability
by phages and the loss of sensitivity to quaternary ammonium compounds
are suggestive of an intrinsic resistance due to modifications in the
cell wall.
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Low Sensitivity of Listeria
monocytogenes to Quaternary Ammonium Compounds
*
Corresponding author. Mailing address: Laboratoire de
Microbiologie, Faculté de Médecine de Tours, 2 bis Bd
Tonnellé, 37032 Tours Cedex, France. Phone: 33 2 47478113. Fax:
33 2 47478530. E-mail: laurent.mereghetti{at}med.univ-tours.fr.
Applied and Environmental Microbiology, November 2000, p. 5083-5086, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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