This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guo, X.
Right arrow Articles by Brackett, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, X.
Right arrow Articles by Brackett, R. E.
Agricola
Right arrow Articles by Guo, X.
Right arrow Articles by Brackett, R. E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2000, p. 5248-5252, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

PCR Detection of Salmonella enterica Serotype Montevideo in and on Raw Tomatoes Using Primers Derived from hilA

Xuan Guo,1 Jinru Chen,1 Larry R. Beuchat,1,* and Robert E. Brackett1,2

Center for Food Safety and Quality Enhancement, Department of Food Science and Technology, The University of Georgia, Griffin, Georgia 30223-1797,1 and Office of Plant, Dairy Foods and Beverages, U.S. Food and Drug Administration, Washington, D.C. 202042

Received 26 June 2000/Accepted 14 September 2000

Salmonellae have been some of the most frequently reported etiological agents in fresh-produce-associated outbreaks of human infections in recent years. PCR assays using four innovative pairs of primers derived from hilA and sirA, positive regulators of Salmonella invasive genes, were developed to identify Salmonella enterica serotype Montevideo on and in tomatoes. Based on examination of 83 Salmonella strains and 22 non-Salmonella strains, we concluded that a pair of hilA primers detects Salmonella specifically. The detection limits of the PCR assay were 101 and 100 CFU/ml after enrichment at 37°C for 6 and 9 h, respectively. When the assay was validated by detecting S. enterica serotype Montevideo in and on artificially inoculated tomatoes, 102 and 101 CFU/g were detected, respectively, after enrichment for 6 h at 37°C. Our results suggest that the hilA-based PCR assay is sensitive and specific, and can be used for rapid detection of Salmonellae in or on fresh produce.

* Corresponding author. Mailing address: Center for Food Safety and Quality Enhancement, Department of Food Science and Technology, The University of Georgia, 1109 Experiment Street, Griffin, GA 30223-1797. Phone: (770) 412-4740. Fax: (770) 229-3216. E-mail: lbeucha{at}cfsqe.griffin.peachnet.edu.

Applied and Environmental Microbiology, December 2000, p. 5248-5252, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Borsoi, A., Santin, E., Santos, L. R., Salle, C. T. P., Moraes, H. L. S., Nascimento, V. P. (2009). Inoculation of newly hatched broiler chicks with two Brazilian isolates of Salmonella Heidelberg strains with different virulence gene profiles, antimicrobial resistance, and pulsed field gel electrophoresis patterns to intestinal changes evaluation. Poult. Sci. 88: 750-758 [Abstract] [Full Text]  
  • Sanchez-Jimenez, M. M., Cardona-Castro, N. (2004). Validation of a PCR for diagnosis of typhoid fever and salmonellosis by amplification of the hilA gene in clinical samples from Colombian patients. J Med Microbiol 53: 875-878 [Abstract] [Full Text]  
  • Guo, X., Chen, J., Brackett, R. E., Beuchat, L. R. (2001). Survival of Salmonellae on and in Tomato Plants from the Time of Inoculation at Flowering and Early Stages of Fruit Development through Fruit Ripening. Appl. Environ. Microbiol. 67: 4760-4764 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»