This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Greene, E. A.
Right arrow Articles by Voordouw, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Greene, E. A.
Right arrow Articles by Voordouw, G.
Agricola
Right arrow Articles by Greene, E. A.
Right arrow Articles by Voordouw, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2000, p. 5282-5289, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Composition of Soil Microbial Communities Enriched on a Mixture of Aromatic Hydrocarbons

E. Anne Greene,1,* Jason G. Kay,1 Karim Jaber,1 Les G. Stehmeier,2 and Gerrit Voordouw1

Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4,1 and NOVA Research and Technology Corporation, Calgary, Alberta T2E 7K7,2 Canada

Received 19 July 2000/Accepted 20 September 2000

Soil contaminated with C5+, which contained benzene (45%, wt/wt), dicyclopentadiene (DCPD) plus cyclopentadiene (together 20%), toluene (6%), styrene (3%), xylenes (2%), naphthalene (2%), and smaller quantities of other compounds, served as the source for isolation of 55 genomically distinct bacteria (standards). Use of benzene as a substrate by these bacteria was most widespread (31 of 44 standards tested), followed by toluene (23 of 44), xylenes (14 of 44), styrene (10 of 44), and naphthalene (10 of 44). Master filters containing denatured genomic DNAs of all 55 standards were used to analyze the community compositions of C5+ enrichment cultures by reverse sample genome probing (RSGP). The communities enriched from three contaminated soils were similar to those enriched from three uncontaminated soils from the same site. The compositions of these communities were time dependent and showed a succession of Pseudomonas and Rhodococcus spp. before convergence on a composition dominated by Alcaligenes spp. The dominant community members detected by RSGP were capable of benzene degradation at all stages of succession. The enrichments effectively degraded all C5+ components except DCPD. Overall, degradation of individual C5+ hydrocarbons followed first-order kinetics, with the highest rates of removal for benzene.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Calgary, 2500 University Dr. NW, Calgary, Alberta T2N 1N4, Canada. Phone: (403) 220-6388. Fax: (403) 289-9311. E-mail: agreene{at}ucalgary.ca.

Applied and Environmental Microbiology, December 2000, p. 5282-5289, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Popp, N., Schlomann, M., Mau, M. (2006). Bacterial diversity in the active stage of a bioremediation system for mineral oil hydrocarbon-contaminated soils.. Microbiology 152: 3291-3304 [Abstract] [Full Text]  
  • Ni Chadhain, S. M., Norman, R. S., Pesce, K. V., Kukor, J. J., Zylstra, G. J. (2006). Microbial Dioxygenase Gene Population Shifts during Polycyclic Aromatic Hydrocarbon Biodegradation.. Appl. Environ. Microbiol. 72: 4078-4087 [Abstract] [Full Text]  
  • Hendrickx, B., Dejonghe, W., Boenne, W., Brennerova, M., Cernik, M., Lederer, T., Bucheli-Witschel, M., Bastiaens, L., Verstraete, W., Top, E. M., Diels, L., Springael, D. (2005). Dynamics of an Oligotrophic Bacterial Aquifer Community during Contact with a Groundwater Plume Contaminated with Benzene, Toluene, Ethylbenzene, and Xylenes: an In Situ Mesocosm Study. Appl. Environ. Microbiol. 71: 3815-3825 [Abstract] [Full Text]  
  • Kaplan, C. W., Kitts, C. L. (2004). Bacterial Succession in a Petroleum Land Treatment Unit. Appl. Environ. Microbiol. 70: 1777-1786 [Abstract] [Full Text]  
  • Liu, L., Coenye, T., Burns, J. L., Whitby, P. W., Stull, T. L., LiPuma, J. J. (2002). Ribosomal DNA-Directed PCR for Identification of Achromobacter (Alcaligenes) xylosoxidans Recovered from Sputum Samples from Cystic Fibrosis Patients. J. Clin. Microbiol. 40: 1210-1213 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»