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Applied and Environmental Microbiology, December 2000, p. 5329-5333, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Anaerobic Degradation of 2-Methylnaphthalene by a Sulfate-Reducing Enrichment Culture†

Eva Annweiler,1 Arne Materna,2 Michael Safinowski,2 Andreas Kappler,2 Hans H. Richnow,3 Walter Michaelis,1 and Rainer U. Meckenstock2,*

Institut für Biogeochemie und Meereschemie, Universität Hamburg, D-20146 Hamburg,1 UFZ, Umweltforschungszentrum Leipzig-Halle GmbH, D-04318 Leipzig,3 and Fakultät für Biologie, Universität Konstanz, D-78457 Konstanz,2 Germany

Received 26 June 2000/Accepted 29 September 2000

Anaerobic degradation of 2-methylnaphthalene was investigated with a sulfate-reducing enrichment culture. Metabolite analyses revealed two groups of degradation products. The first group comprised two succinic acid adducts which were identified as naphthyl-2-methyl-succinic acid and naphthyl-2-methylene-succinic acid by comparison with chemically synthesized reference compounds. Naphthyl-2-methyl-succinic acid accumulated to 0.5 µM in culture supernatants. Production of naphthyl-2-methyl-succinic acid was analyzed in enzyme assays with dense cell suspensions. The conversion of 2-methylnaphthalene to naphthyl-2-methyl-succinic acid was detected at a specific activity of 0.020 ± 0.003 nmol min-1 mg of protein-1 only in the presence of cells and fumarate. We conclude that under anaerobic conditions 2-methylnaphthalene is activated by fumarate addition to the methyl group, as is the case in anaerobic toluene degradation. The second group of metabolites comprised 2-naphthoic acid and reduced 2-naphthoic acid derivatives, including 5,6,7,8-tetrahydro-2-naphthoic acid, octahydro-2-naphthoic acid, and decahydro-2-naphthoic acid. These compounds were also identified in an earlier study as products of anaerobic naphthalene degradation with the same enrichment culture. A pathway for anaerobic degradation of 2-methylnaphthalene analogous to that for anaerobic toluene degradation is proposed.

* Corresponding author. Mailing address: Fakultät für Biologie, Universität Konstanz, Universitätsstr. 10, D-78457 Konstanz, Germany. Phone: 49-7531-884541. Fax: 49-7531-882966. E-mail: rainer.meckenstock{at}uni-konstanz.de.

dagger Publication 101 of the Deutsche Forschungsgemeinschaft priority program 546, Geochemical Processes with Long-Term Effects in Anthropogenically Affected Seepage and Groundwater.

Applied and Environmental Microbiology, December 2000, p. 5329-5333, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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