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Applied and Environmental Microbiology, December 2000, p. 5457-5459, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid Dye Decolorization Method for Screening Potential Wood Preservatives

Olga Borokhov* and Stephen Rothenburgerdagger

Lonzagroup, Research and Development Laboratories, Annandale, New Jersey 08801

Received 24 April 2000/Accepted 9 September 2000

We developed a new screening method for potential wood preservatives based on decolorization of the dye Remazol Brilliant Blue R by extracellular oxidative agents produced by wood decay fungi. Oxidative biodegradation of lignin yielded decolorized zones around and under fungal cultures on a dyed agar medium. Inhibitory effects were detected by direct observation and measurement of the decolorized zones.


* Corresponding author. Mailing address: Lonzagroup, Research and Development Laboratories, 79 Route 22 East, P.O. Box 993, Annandale, NJ 08801. Phone: (908) 730-1553. Fax: (908) 730-1546. E-mail: oborokhov{at}lonza-us.com.

dagger Present address: Ethicon, Inc., Somerville, NJ 08876-0151.


Applied and Environmental Microbiology, December 2000, p. 5457-5459, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.