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Applied and Environmental Microbiology, December 2000, p. 5469-5471, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Bradyrhizobium japonicum Proline Biosynthesis Gene proC Is Essential for Symbiosis

Natalie D. King, David Hojnacki, and Mark R. O'Brian*

Department of Biochemistry and Center for Microbial Pathogenesis, State University of New York at Buffalo, Buffalo, New York 14214

Received 29 June 2000/Accepted 15 September 2000

Plant host-derived proline is proposed to serve as an energy source for rhizobia in the rhizosphere and in symbiotic root nodules. The Bradyrhizobium japonicum proC gene was isolated, and a proC mutant strain that behaved as a strict proline auxotroph in culture was constructed. The proC strain elicited undeveloped nodules on soybeans that lacked nitrogen fixation activity and plant hemoglobin. We conclude that the proC gene is essential for symbiosis and suggest that the mutant does not obtain an exogenous supply of proline in association with soybeans sufficient to satisfy its auxotrophy.

* Corresponding author. Mailing address: Department of Biochemistry, 140 Farber Hall, State University of New York at Buffalo, Buffalo, NY 14214. Phone: (716)829-3200. Fax: (716)829-2725. E-mail: mrobrian{at}buffalo.edu.

Applied and Environmental Microbiology, December 2000, p. 5469-5471, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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