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Applied and Environmental Microbiology, December 2000, p. 5477-5479, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Improved Secretion of Native Human Insulin-Like Growth Factor 1 from gas1 Mutant Saccharomyces cerevisiae Cells

Marina Vai,1 Luca Brambilla,1 Ivan Orlandi,1 Nicola Rota,2 Bianca Maria Ranzi,2 Lilia Alberghina,1 and Danilo Porro1,*

Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, 20126 Milan,1 and Dipartimento di Fisiologia e Biochimica Generali, Università degli Studi di Milano, 20133 Milan,2 Italy

Received 2 May 2000/Accepted 8 September 2000

We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor alpha  prepro- leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.

* Corresponding author. Mailing address: Università degli Studi di Milano-Bicocca, Dipartimento di Biotecnologie e Bioscienze, P.za della Scienza 2, 20126 Milan, Italy. Phone: 39 02 64483451. Fax: 39 02 64483565. E-mail:danilo.porro{at}unimib.it.

Applied and Environmental Microbiology, December 2000, p. 5477-5479, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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