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Applied and Environmental Microbiology, December 2000, p. 5492-5498, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of Species and Sources of Cryptosporidium Oocysts in Storm Waters with a Small-Subunit rRNA-Based Diagnostic and Genotyping Tool

Lihua Xiao,1,* Kerri Alderisio,2 Josef Limor,1 Michael Royer,3 and Altaf A. Lal1

Division of Parasitic Diseases, Centers for Disease Control and Prevention, Chamblee, Georgia 303411; Division of Drinking Water Quality Control, New York City Department of Environmental Protection, Valhalla, New York 105952; and Urban Watershed Management Branch, Water Supply and Water Resources Division, U.S. Environmental Protection Agency, Edison, New Jersey 088373

Received 31 May 2000/Accepted 7 September 2000

The identification of Cryptosporidium oocysts in environmental samples is largely made by the use of an immunofluorescent assay. In this study, we have used a small-subunit rRNA-based PCR-restriction fragment length polymorphism technique to identify species and sources of Cryptosporidium oocysts present in 29 storm water samples collected from a stream in New York. A total of 12 genotypes were found in 27 positive samples; for 4 the species and probable origins were identified by sequence analysis, whereas the rest represent new genotypes from wildlife. Thus, this technique provides an alternative method for the detection and differentiation of Cryptosporidium parasites in environmental samples.

* Corresponding author. Mailing address: Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770 Buford Highway, Atlanta, GA 30341-3717. Phone: (770) 488-4840. Fax: (770) 488-4454. E-mail: LAX0{at}CDC.GOV.

Applied and Environmental Microbiology, December 2000, p. 5492-5498, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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