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Applied and Environmental Microbiology, December 2000, p. 5503-5505, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Anaerobic Toluene Activation by Benzylsuccinate Synthase in a Highly Enriched Methanogenic Culture

Harry R. Beller1,* and Elizabeth A. Edwards2

Lawrence Livermore National Laboratory, Livermore, California 94551-0808,1 and Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada2

Received 11 July 2000/Accepted 2 October 2000

Permeabilized cells of a highly enriched, toluene-mineralizing, methanogenic culture catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. The specific in vitro rate of benzylsuccinate formation was >85% of the specific in vivo rate of toluene consumption. This is the first report of benzylsuccinate synthase activity in a methanogenic culture; the activity has previously been reported to occur in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria.

* Corresponding author. Mailing address: Lawrence Livermore National Laboratory, P.O. Box 808, L-542, Livermore, CA 94551-0808. Phone: (925) 422-0081. Fax: (925) 423-7998. E-mail: beller2{at}llnl.gov.

Applied and Environmental Microbiology, December 2000, p. 5503-5505, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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