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Applied and Environmental Microbiology, December 2000, p. 5518-5520, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Diacetyl and alpha -Acetolactate Overproduction by Lactococcus lactis subsp. lactis Biovar Diacetylactis Mutants That Are Deficient in alpha -Acetolactate Decarboxylase and Have a Low Lactate Dehydrogenase Activity

Christophe Monnet,* Frédéric Aymes,dagger and Georges Corrieu

Laboratoire de Génie et Microbiologie des Procédés Alimentaires, Institut National de la Recherche Agronomique, 78850 Thiverval-Grignon, France

Received 28 June 2000/Accepted 25 September 2000

Lactococcus lactis subsp. lactis biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha -acetolactate. Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in alpha -acetolactate decarboxylase and had low lactate dehydrogenase activity. The mutants produced large amounts of alpha -acetolactate in anaerobic milk cultures but not in aerobic cultures, except when the medium was supplemented with catalase, yeast extract, or hemoglobin.

* Corresponding author. Mailing address: INRA, LGMPA, 78850 Thiverval-Grignon, France. Phone: 33 1 30 81 54 91. Fax: 33 1 30 81 55 97. E-mail: monnet{at}grignon.inra.fr.

dagger Present address: Nestlé Product Technology Centre, Beuvillers, 14100 Lisieux, France.

Applied and Environmental Microbiology, December 2000, p. 5518-5520, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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