This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yokomaku, D.
Right arrow Articles by Nasu, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yokomaku, D.
Right arrow Articles by Nasu, M.
Agricola
Right arrow Articles by Yokomaku, D.
Right arrow Articles by Nasu, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2000, p. 5544-5548, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Improved Direct Viable Count Procedure for Quantitative Estimation of Bacterial Viability in Freshwater Environments

Daisaku Yokomaku, Nobuyasu Yamaguchi, and Masao Nasu*

Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan

Received 25 May 2000/Accepted 11 September 2000

A direct viable count (DVC) procedure was developed which clearly and easily discriminates the viability of bacterial cells. In this quantitative DVC (qDVC) procedure, viable cells are selectively lysed by spheroplast formation caused by incubation with antibiotics and glycine. This glycine effect leads to swollen cells with a very loose cell wall. The viable cells then are lysed easily by a single freeze-thaw treatment. The number of viable cells was obtained by subtracting the number of remaining cells after the qDVC procedure from the total cell number before the qDVC incubation. This improved procedure should provide useful information about the metabolic potential of natural bacterial communities.

* Corresponding author. Mailing address: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-8170. Fax: 81-6-6879-8174. E-mail: nasu{at}phs.osaka-u.ac.jp.

Applied and Environmental Microbiology, December 2000, p. 5544-5548, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Falcioni, T., Papa, S., Gasol, J. M. (2008). Evaluating the Flow-Cytometric Nucleic Acid Double-Staining Protocol in Realistic Situations of Planktonic Bacterial Death. Appl. Environ. Microbiol. 74: 1767-1779 [Abstract] [Full Text]  
  • Nocker, A., Sossa-Fernandez, P., Burr, M. D., Camper, A. K. (2007). Use of Propidium Monoazide for Live/Dead Distinction in Microbial Ecology. Appl. Environ. Microbiol. 73: 5111-5117 [Abstract] [Full Text]  
  • Yamaguchi, N., Baba, T., Nakagawa, S., Saito, A., Nasu, M. (2007). Rapid monitoring of bacteria in dialysis fluids by fluorescent vital staining and microcolony methods. Nephrol Dial Transplant 22: 612-616 [Full Text]  
  • Maruyama, F., Tani, K., Kenzaka, T., Yamaguchi, N., Nasu, M. (2006). Quantitative Determination of Free-DNA Uptake in River Bacteria at the Single-Cell Level by In Situ Rolling-Circle Amplification. Appl. Environ. Microbiol. 72: 6248-6256 [Abstract] [Full Text]  
  • Porter, J., Morris, S. A., Pickup, R. W. (2004). Effect of Trophic Status on the Culturability and Activity of Bacteria from a Range of Lakes in the English Lake District. Appl. Environ. Microbiol. 70: 2072-2078 [Abstract] [Full Text]  
  • Baudart, J., Coallier, J., Laurent, P., Prevost, M. (2002). Rapid and Sensitive Enumeration of Viable Diluted Cells of Members of the Family Enterobacteriaceae in Freshwater and Drinking Water. Appl. Environ. Microbiol. 68: 5057-5063 [Abstract] [Full Text]  
  • Luna, G. M., Manini, E., Danovaro, R. (2002). Large Fraction of Dead and Inactive Bacteria in Coastal Marine Sediments: Comparison of Protocols for Determination and Ecological Significance. Appl. Environ. Microbiol. 68: 3509-3513 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»