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Applied and Environmental Microbiology, February 2000, p. 783-787, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Immunolocalization of Dinitrogenase Reductase Produced by Klebsiella pneumoniae in Association with Zea mays L.

Marisa K. Chelius and Eric W. Triplett*

Center for the Study of Nitrogen Fixation, Brock Institute for Environmental Microbiology, and Department of Agronomy, University of Wisconsin-Madison, Madison, Wisconsin 53706

Received 30 August 1999/Accepted 15 November 1999

The endophytic lifestyle of Klebsiella pneumoniae is described, including the production of dinitrogenase reductase by bacteria residing in maize root tissue. The green fluorescent protein (GFP) was used to detect the colonization of maize by K. pneumoniae strains 2028 and 342. These strains were found to reside in intercortical layers of the stem and within the region of maturation in the root. The production of dinitrogenase reductase by GFP-tagged bacteria was visualized using immunolocalization. This activity was only apparent when bacteria were supplied with an exogenous carbon source. The results suggest that maize provides a suitable habitat for K. pneumoniae and that this species is capable of producing nitrogenase under the appropriate plant cultivation conditions.

* Corresponding author. Mailing address: Department of Agronomy, University of Wisconsin-Madison, Madison, WI 53706-1597. Phone: (608) 262-9824. Fax: (608) 262-5217. E-mail: triplett{at}facstaff.wisc.edu.

Applied and Environmental Microbiology, February 2000, p. 783-787, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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