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Applied and Environmental Microbiology, February 2000, p. 836-838, Vol. 66, No. 2
Center for Biofilm Engineering,1
Department of Chemical Engineering,2
Department of Land Resources and Environmental
Science,3 and Department of Civil
Engineering,4 Montana State University
Received 24 June 1999/Accepted 9 November 1999
The penetration of hydrogen peroxide into biofilms formed by
wild-type and catalase-deficient Pseudomonas aeruginosa
strains was measured using microelectrodes. A flowing stream of
hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill
wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked
out. Catalase protects aggregated bacteria by preventing full
penetration of hydrogen peroxide into the biofilm.
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Effect of Catalase on Hydrogen Peroxide Penetration
into Pseudomonas aeruginosa Biofilms
Bozeman,
Bozeman, Montana 59717-3980; Department of Microbiology,
University of Colorado Health Sciences Center, Denver, Colorado
802625; and Department of Molecular
Genetics, Biochemistry, and Microbiology, University of Cincinnati
College of Medicine, Cincinnati, Ohio 45257-05246
*
Corresponding author. Mailing address: Center for
Biofilm Engineering, 366 EPS Building, Montana State University,
Bozeman, MT 59717-3980. Phone: (406) 994-2890. Fax: (406) 994-6098. E-mail: phil_s{at}erc.montana.edu.
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