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Applied and Environmental Microbiology, February 2000, p. 850-854, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pathovars of Pseudomonas syringae Causing Bacterial Brown Spot and Halo Blight in Phaseolus vulgaris L. Are Distinguishable by Ribotyping

Ana J. González,1,* Elena Landeras,2 and M. Carmen Mendoza3

Servicio Regional de Investigación y Desarrollo Agrario, Consejería de Agricultura, Ganadería y Pesca, 33300-Villaviciosa,1 Laboratorio de Sanidad Vegetal, Consejería de Agricultura, Ganadería y Pesca, 33071-Oviedo,2 and Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006-Oviedo,3 Principado de Asturias, Spain

Received 26 July 1999/Accepted 19 November 1999

Ribotyping was evaluated as a method to differentiate between Pseudomonas syringae pv. phaseolicola and pv. syringae strains causing bacterial brown spot and halo blight diseases in Phaseolus vulgaris L. Ribotyping, with restriction enzymes BglI and SalI and using the Escherichia coli rrnB operon as the probe, differentiated 11 and 14 ribotypes, respectively, and a combination of data from both procedures yielded 19 combined ribotypes. Cluster analysis of the combined ribotypes differentiated the pathovars phaseolicola and syringae, as well as different clonal lineages within these pathovars. The potential of ribotyping to screen for correlations between lineages and factors such as geographical region and/or bean varieties is also reported.

* Corresponding author. Mailing address: SERIDA, Apartado 13, 33300 Villaviciosa, Asturias, Spain. Phone: 34-985890066. Fax: 34-985891854. E-mail: anagf{at}princast.es.

Applied and Environmental Microbiology, February 2000, p. 850-854, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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