This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by González, A. J.
Right arrow Articles by Mendoza, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by González, A. J.
Right arrow Articles by Mendoza, M. C.
Agricola
Right arrow Articles by González, A. J.
Right arrow Articles by Mendoza, M. C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2000, p. 850-854, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pathovars of Pseudomonas syringae Causing Bacterial Brown Spot and Halo Blight in Phaseolus vulgaris L. Are Distinguishable by Ribotyping

Ana J. González,1,* Elena Landeras,2 and M. Carmen Mendoza3

Servicio Regional de Investigación y Desarrollo Agrario, Consejería de Agricultura, Ganadería y Pesca, 33300-Villaviciosa,1 Laboratorio de Sanidad Vegetal, Consejería de Agricultura, Ganadería y Pesca, 33071-Oviedo,2 and Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, 33006-Oviedo,3 Principado de Asturias, Spain

Received 26 July 1999/Accepted 19 November 1999

Ribotyping was evaluated as a method to differentiate between Pseudomonas syringae pv. phaseolicola and pv. syringae strains causing bacterial brown spot and halo blight diseases in Phaseolus vulgaris L. Ribotyping, with restriction enzymes BglI and SalI and using the Escherichia coli rrnB operon as the probe, differentiated 11 and 14 ribotypes, respectively, and a combination of data from both procedures yielded 19 combined ribotypes. Cluster analysis of the combined ribotypes differentiated the pathovars phaseolicola and syringae, as well as different clonal lineages within these pathovars. The potential of ribotyping to screen for correlations between lineages and factors such as geographical region and/or bean varieties is also reported.

* Corresponding author. Mailing address: SERIDA, Apartado 13, 33300 Villaviciosa, Asturias, Spain. Phone: 34-985890066. Fax: 34-985891854. E-mail: anagf{at}princast.es.

Applied and Environmental Microbiology, February 2000, p. 850-854, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Rivas, L. A., Mansfield, J., Tsiamis, G., Jackson, R. W., Murillo, J. (2005). Changes in Race-Specific Virulence in Pseudomonas syringae pv. phaseolicola Are Associated with a Chimeric Transposable Element and Rare Deletion Events in a Plasmid-Borne Pathogenicity Island. Appl. Environ. Microbiol. 71: 3778-3785 [Abstract] [Full Text]  
  • Oguiza, J. A., Rico, A., Rivas, L. A., Sutra, L., Vivian, A., Murillo, J. (2004). Pseudomonas syringae pv. phaseolicola can be separated into two genetic lineages distinguished by the possession of the phaseolotoxin biosynthetic cluster. Microbiology 150: 473-482 [Abstract] [Full Text]  
  • Gonzalez, A. J., Rodicio, M. R., Mendoza, M. C. (2003). Identification of an Emergent and Atypical Pseudomonas viridiflava Lineage Causing Bacteriosis in Plants of Agronomic Importance in a Spanish Region. Appl. Environ. Microbiol. 69: 2936-2941 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»