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Applied and Environmental Microbiology, February 2000, p. 869-873, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Survival of Bifidobacterium longum Immobilized in Calcium Alginate Beads in Simulated Gastric Juices and Bile Salt Solution

Ki-Yong Lee and Tae-Ryeon Heo*

Department of Biological Engineering, Inha University, Inchon, Korea

Received 3 May 1999/Accepted 10 November 1999

Bifidobacterium longum KCTC 3128 and HLC 3742 were independently immobilized (entrapped) in calcium alginate beads containing 2, 3, and 4% sodium alginate. When the bifidobacteria entrapped in calcium alginate beads were exposed to simulated gastric juices and a bile salt solution, the death rate of the cells in the beads decreased proportionally with an increase in both the alginate gel concentration and bead size. The initial cell numbers in the beads affected the numbers of survivors after exposure to these solutions; however, the death rates of the viable cells were not affected. Accordingly, a mathematical model was formulated which expressed the influences of several parameters (gel concentration, bead size, and initial cell numbers) on the survival of entrapped bifidobacteria after sequential exposure to simulated gastric juices followed by a bile salt solution. The model proposed in this paper may be useful for estimating the survival of bifidobacteria in beads and establishing optimal entrapment conditions.

* Corresponding author. Mailing address: Postal Code 402-751, Department of Biological Engineering, Inha University, Yonghyun-dong 253, Nam-gu, Inchon, Korea. Phone: 82-032-860-7511. Fax: 82-032-873-4429. E-mail: heotary{at}dragon.inha.ac.kr.

Applied and Environmental Microbiology, February 2000, p. 869-873, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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