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Applied and Environmental Microbiology, March 2000, p. 1084-1092, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization and Determination of Origin of Lactic Acid Bacteria from a Sorghum-Based Fermented Weaning Food by Analysis of Soluble Proteins and Amplified Fragment Length Polymorphism Fingerprinting

Nokuthula F. Kunene,1 Ifigenia Geornaras,2 Alexander von Holy,2 and John W. Hastings1,*

School of Molecular and Cellular Biosciences, University of Natal, Scottsville, 3209,1 and Department of Molecular and Cell Biology, University of the Witwatersrand, Wits 2050,2 South Africa

Received 12 July 1999/Accepted 26 November 1999

The group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known, and these organisms have been characterized extensively by using different techniques. In this study, 180 lactic acid bacterial strains isolated from sorghum powder (44 strains) and from corresponding fermented (93 strains) and cooked fermented (43 strains) porridge samples that were prepared in 15 households were characterized by using biochemical and physiological methods, as well as by analyzing the electrophoretic profiles of total soluble proteins. A total of 58 of the 180 strains were Lactobacillus plantarum strains, 47 were Leuconostoc mesenteroides strains, 25 were Lactobacillus sake-Lactobacillus curvatus strains, 17 were Pediococcus pentosaceus strains, 13 were Pediococcus acidilactici strains, and 7 were Lactococcus lactis strains. L. plantarum and L. mesenteroides strains were the dominant strains during the fermentation process and were recovered from 87 and 73% of the households, respectively. The potential origins of these groups of lactic acid bacteria were assessed by amplified fragment length polymorphism fingerprint analysis.

* Corresponding author. Mailing address: School of Molecular and Cellular Biosciences, University of Natal, Private Bag X01, Scottsville 3209, South Africa. Phone: 27 331 260 5434. Fax: 27 331 260 5435. E-mail: hastings{at}gene.unp.ac.za.

Applied and Environmental Microbiology, March 2000, p. 1084-1092, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Geornaras, I., Hastings, J. W., von Holy, A. (2001). Genotypic Analysis of Escherichia coli Strains from Poultry Carcasses and Their Susceptibilities to Antimicrobial Agents. Appl. Environ. Microbiol. 67: 1940-1944 [Abstract] [Full Text]  


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