This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coconnier, M.-H.
Right arrow Articles by Servin, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coconnier, M.-H.
Right arrow Articles by Servin, A. L.
Agricola
Right arrow Articles by Coconnier, M.-H.
Right arrow Articles by Servin, A. L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2000, p. 1152-1157, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antagonistic Activity of Lactobacillus acidophilus LB against Intracellular Salmonella enterica Serovar Typhimurium Infecting Human Enterocyte-Like Caco-2/TC-7 Cells

Marie-Hélène Coconnier, Vanessa Liévin, Mathie Lorrot, and Alain L. Servin*

Pathogènes et Fonctions des Cellules Epithéliales Polarisées, Institut National de la Santé et de la Recherche Médicale, Unité 510, Faculté de Pharmacie, Université Paris XI, F-92296 Châtenay-Malabry, France

Received 22 November 1999/Accepted 16 December 1999

To gain further insight into the mechanism by which lactobacilli develop antimicrobial activity, we have examined how Lactobacillus acidophilus LB inhibits the promoted cellular injuries and intracellular lifestyle of Salmonella enterica serovar Typhimurium SL1344 infecting the cultured, fully differentiated human intestinal cell line Caco-2/TC-7. We showed that the spent culture supernatant of strain LB (LB-SCS) decreases the number of apical serovar Typhimurium-induced F-actin rearrangements in infected cells. LB-SCS treatment efficiently decreased transcellular passage of S. enterica serovar Typhimurium. Moreover, LB-SCS treatment inhibited intracellular growth of serovar Typhimurium, since treated intracellular bacteria displayed a small, rounded morphology resembling that of resting bacteria. We also showed that LB-SCS treatment inhibits adhesion-dependent serovar Typhimurium-induced interleukin-8 production.

* Corresponding author. Mailing address: Unité 510, Faculté de Pharmacie, Université Paris XI, F-92296 Châtenay-Malabry, France. Phone: (33) 1.46.83.56.61. Fax: (33) 1.46.83.56.61. E-mail: alain.servin{at}cep.u-psud.fr.

Applied and Environmental Microbiology, March 2000, p. 1152-1157, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Prado Acosta, M., Palomino, M. M., Allievi, M. C., Rivas, C. S., Ruzal, S. M. (2008). Murein Hydrolase Activity in the Surface Layer of Lactobacillus acidophilus ATCC 4356. Appl. Environ. Microbiol. 74: 7824-7827 [Abstract] [Full Text]  
  • Wagner, R. D., Johnson, S. J., Cerniglia, C. E. (2008). In Vitro Model of Colonization Resistance by the Enteric Microbiota: Effects of Antimicrobial Agents Used in Food-Producing Animals. Antimicrob. Agents Chemother. 52: 1230-1237 [Abstract] [Full Text]  
  • Lievin-Le Moal, V., Sarrazin-Davila, L. E., Servin, A. L. (2007). An Experimental Study and a Randomized, Double-Blind, Placebo-Controlled Clinical Trial to Evaluate the Antisecretory Activity of Lactobacillus acidophilus Strain LB Against Nonrotavirus Diarrhea. Pediatrics 120: e795-e803 [Abstract] [Full Text]  
  • Lievin-Le Moal, V., Servin, A. L. (2006). The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota. Clin. Microbiol. Rev. 19: 315-337 [Abstract] [Full Text]  
  • Buck, B. L., Altermann, E., Svingerud, T., Klaenhammer, T. R. (2005). Functional Analysis of Putative Adhesion Factors in Lactobacillus acidophilus NCFM. Appl. Environ. Microbiol. 71: 8344-8351 [Abstract] [Full Text]  
  • Coconnier-Polter, M.-H., Lievin-Le Moal, V., Servin, A. L. (2005). A Lactobacillus acidophilus Strain of Human Gastrointestinal Microbiota Origin Elicits Killing of Enterovirulent Salmonella enterica Serovar Typhimurium by Triggering Lethal Bacterial Membrane Damage. Appl. Environ. Microbiol. 71: 6115-6120 [Abstract] [Full Text]  
  • Ingrassia, I., Leplingard, A., Darfeuille-Michaud, A. (2005). Lactobacillus casei DN-114 001 Inhibits the Ability of Adherent-Invasive Escherichia coli Isolated from Crohn's Disease Patients To Adhere to and To Invade Intestinal Epithelial Cells. Appl. Environ. Microbiol. 71: 2880-2887 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»