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Applied and Environmental Microbiology, March 2000, p. 1237-1242, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Transformation of Acinetobacter sp. Strain BD413(pFG4Delta nptII) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants

Kaare M. Nielsen,1,* Jan D. van Elsas,2 and Kornelia Smalla3

Unigen and Department of Botany, Norwegian University of Science and Technology, 7491 Trondheim, Norway1; Research Institute for Plant Protection, IPO-DLO, 6700 GW Wageningen, The Netherlands2; and Institute for Plant Virology, Microbiology and Biosafety, Federal Biological Research Centre for Agriculture and Forestry, (BBA), 38104 Braunschweig, Germany3

Received 1 November 1999/Accepted 27 December 1999

Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptII gene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant DNA encoding a functional nptII gene conferring bacterial kanamycin resistance. Selective effects of the addition of kanamycin on the population dynamics of Acinetobacter sp. cells in soil were found, and high concentrations of kanamycin reduced the CFU of Acinetobacter sp. cells from 109 CFU/g of soil to below detection. In contrast to a chromosomal nptII-encoded kanamycin resistance, the pFG4-generated resistance was found to be unstable over a 31-day incubation period in vitro.

* Corresponding author. Present address: D. Hartl Laboratory, Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138. Phone: (617) 496-5540. Fax: (617) 496-5854. E-mail: knielsen{at}oeb.harvard.edu.

Applied and Environmental Microbiology, March 2000, p. 1237-1242, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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