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Applied and Environmental Microbiology, March 2000, p. 1243-1248, Vol. 66, No. 3
Department of Biotechnology1 and
Department of Analytical Chemistry,2
Kungliga Tekniska Högskolan, S-100 44 Stockholm, Sweden
Received 12 October 1999/Accepted 20 December 1999
Recombinant Staphylococcus xylosus and
Staphylococcus carnosus strains were generated with
surface-exposed chimeric proteins containing polyhistidyl peptides
designed for binding to divalent metal ions. Surface accessibility of
the chimeric surface proteins was demonstrated and the chimeric surface
proteins were found to be functional in terms of metal binding, since
the recombinant staphylococcal cells were shown to have gained
Ni2+- and Cd2+-binding capacity, suggesting
that such bacteria could find use in bioremediation of heavy metals.
This is, to our knowledge, the first time that recombinant,
surface-exposed metal-binding peptides have been expressed on
gram-positive bacteria. Potential environmental or biosensor
applications for such recombinant staphylococci as biosorbents are discussed.
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Staphylococcal Surface Display of Metal-Binding
Polyhistidyl Peptides
*
Corresponding author. Mailing address: Department of
Biotechnology, Kungliga Tekniska Högskolan, S-100 44 Stockholm,
Sweden. Phone: 46 8 790 6497. Fax: 46 8 245452. E-mail:
stefans{at}biochem.kth.se.
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