This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Samuelson, P. Articles by Ståhl, S. Search for Related Content PubMed PubMed Citation Articles by Samuelson, P. Articles by Ståhl, S. Agricola Articles by Samuelson, P. Articles by Ståhl, S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2000, p. 1243-1248, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Staphylococcal Surface Display of Metal-Binding Polyhistidyl Peptides

Patrik Samuelson,¹ Henrik Wernérus,¹ Malin Svedberg,² and Stefan Ståhl^1,*

Department of Biotechnology¹ and Department of Analytical Chemistry,² Kungliga Tekniska Högskolan, S-100 44 Stockholm, Sweden

Received 12 October 1999/Accepted 20 December 1999

Recombinant Staphylococcus xylosus and Staphylococcus carnosus strains were generated with surface-exposed chimeric proteins containing polyhistidyl peptides designed for binding to divalent metal ions. Surface accessibility of the chimeric surface proteins was demonstrated and the chimeric surface proteins were found to be functional in terms of metal binding, since the recombinant staphylococcal cells were shown to have gained Ni²⁺- and Cd²⁺-binding capacity, suggesting that such bacteria could find use in bioremediation of heavy metals. This is, to our knowledge, the first time that recombinant, surface-exposed metal-binding peptides have been expressed on gram-positive bacteria. Potential environmental or biosensor applications for such recombinant staphylococci as biosorbents are discussed.

---

^* Corresponding author. Mailing address: Department of Biotechnology, Kungliga Tekniska Högskolan, S-100 44 Stockholm, Sweden. Phone: 46 8 790 6497. Fax: 46 8 245452. E-mail: stefans{at}biochem.kth.se.

---

Applied and Environmental Microbiology, March 2000, p. 1243-1248, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Okano, K., Zhang, Q., Kimura, S., Narita, J., Tanaka, T., Fukuda, H., Kondo, A. (2008). System Using Tandem Repeats of the cA Peptidoglycan-Binding Domain from Lactococcus lactis for Display of both N- and C-Terminal Fusions on Cell Surfaces of Lactic Acid Bacteria. Appl. Environ. Microbiol. 74: 1117-1123 [Abstract] [Full Text]  
• Lofblom, J., Sandberg, J., Wernerus, H., Stahl, S. (2007). Evaluation of Staphylococcal Cell Surface Display and Flow Cytometry for Postselectional Characterization of Affinity Proteins in Combinatorial Protein Engineering Applications. Appl. Environ. Microbiol. 73: 6714-6721 [Abstract] [Full Text]  
• Shoseyov, O., Shani, Z., Levy, I. (2006). Carbohydrate Binding Modules: Biochemical Properties and Novel Applications. Microbiol. Mol. Biol. Rev. 70: 283-295 [Abstract] [Full Text]  
• Ueki, T., Sakamoto, Y., Yamaguchi, N., Michibata, H. (2003). Bioaccumulation of Copper Ions by Escherichia coli Expressing Vanabin Genes from the Vanadium-Rich Ascidian Ascidia sydneiensis samea. Appl. Environ. Microbiol. 69: 6442-6446 [Abstract] [Full Text]  
• Wernerus, H., Samuelson, P., Stahl, S. (2003). Fluorescence-Activated Cell Sorting of Specific Affibody-Displaying Staphylococci. Appl. Environ. Microbiol. 69: 5328-5335 [Abstract] [Full Text]  
• Wernerus, H., Lehtio, J., Teeri, T., Nygren, P.-A., Stahl, S. (2001). Generation of Metal-Binding Staphylococci through Surface Display of Combinatorially Engineered Cellulose-Binding Domains. Appl. Environ. Microbiol. 67: 4678-4684 [Abstract] [Full Text]