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Applied and Environmental Microbiology, March 2000, p. 877-883, Vol. 66, No. 3
0099-2240/00/$04.00+0

Leaching of Escherichia coli O157:H7 in Diverse Soils under Various Agricultural Management Practices

Joel V. Gagliardi* and Jeffrey S. Karns

USDA, Agricultural Research Service, Soil Microbial Systems Laboratory, Beltsville, Maryland 20705

Received 12 August 1999/Accepted 6 December 1999

Application of animal manures to soil as crop fertilizers is an important means for recycling the nitrogen and phosphorus which the manures contain. Animal manures also contain bacteria, including many types of pathogens. Manure pathogen levels depend on the source animal, the animal's state of health, and how the manure was stored or treated before use. Rainfall may result in pathogen spread into soil by runoff from stored or unincorporated manure or by leaching through the soil profile. Steady rainfall consisting of 16.5 mm h-1 was applied to 100-mm disturbed soil cores that were treated with manure and inoculated with Escherichia coli O157:H7 strain B6914. The level of B6914 in leachate was near the inoculum level each hour for 8 h, as was the level of B6914 at several soil depths after 24 h, indicating that there was a high rate of growth. Bacterial movement through three different types of soil was then compared by using disturbed (tilled) and intact (no-till) soil cores and less intense rainfall consisting of 25.4 mm on 4 consecutive days and then four more times over a 17-day period. Total B6914 levels exceeded the inoculum levels for all treatments except intact clay loam cores. B6914 levels in daily leachate samples decreased sharply with time, although the levels were more constant when intact sandy loam cores were used. The presence of manure often increased total B6914 leachate and soil levels in intact cores but had the opposite effect on disturbed soil cores. Ammonia and nitrate levels correlated with B6914 and total coliform levels in leachate. We concluded that tillage practice, soil type, and method of pathogen delivery affect but do not prevent vertical E. coli O157:H7 and coliform transport in soil and that soluble nitrogen may enhance transport.

* Corresponding author. Mailing address: USDA, ARS, SMSL, 10300 Baltimore Avenue, BARC-WEST, Building 001, Room 140, Beltsville, MD 20705. Phone: (301) 504-9214, ext. 332. Fax: (301) 504-8370. E-mail: jgagliar{at}asrr.arsusda.gov.

Applied and Environmental Microbiology, March 2000, p. 877-883, Vol. 66, No. 3
0099-2240/00/$04.00+0


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