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Applied and Environmental Microbiology, March 2000, p. 995-1000, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

High-Affinity Maltose Binding and Transport by the Thermophilic Anaerobe Thermoanaerobacter ethanolicus 39Edagger

C. R. Jones, M. Ray, K. A. Dawson, and H. J. Strobel*

Department of Animal Sciences, University of Kentucky, Lexington, Kentucky 40546-0215

Received 30 August 1999/Accepted 12 December 1999

Thermoanaerobacter ethanolicus is a gram-positive thermophile that produces considerable amounts of ethanol from soluble sugars and polymeric substrates, including starch. Growth on maltose, a product of starch hydrolysis, was associated with the production of a prominent membrane-associated protein that had an apparent molecular weight of 43,800 and was not detected in cells grown on xylose or glucose. Filter-binding assays revealed that cell membranes bound maltose with high affinity. Metabolic labeling of T. ethanolicus maltose-grown cells with [14C]palmitic acid showed that this protein was posttranslationally acylated. A maltose-binding protein was purified by using an amylose resin affinity column, and the binding constant was 270 nM. Since maltase activity was found only in the cytosol of fractionated cells and unlabeled glucose did not compete with radiolabeled maltose for uptake in whole cells, it appeared that maltose was transported intact. In whole-cell transport assays, the affinity for maltose was approximately 40 nM. Maltotriose and alpha -trehalose competitively inhibited maltose uptake in transport assays, whereas glucose, cellobiose, and a range of disaccharides had little effect. Based on these results, it appears that T. ethanolicus possesses a high-affinity, ABC type transport system that is specific for maltose, maltotriose, and alpha -trehalose.

* Corresponding author. Mailing address: 212 W. P. Garrigus Building, Department of Animal Sciences, University of Kentucky, Lexington, KY 40546-0215. Phone: (606) 257-7554. Fax: (606) 257-5318. E-mail: strobel{at}pop.uky.edu.

dagger Published with the approval of the Director of the Kentucky Agricultural Experiment Station as Journal article no. 00-07-7.

Applied and Environmental Microbiology, March 2000, p. 995-1000, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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