This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sharma, N. K.
Right arrow Articles by Dodd, C. E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sharma, N. K.
Right arrow Articles by Dodd, C. E. R.
Agricola
Right arrow Articles by Sharma, N. K.
Right arrow Articles by Dodd, C. E. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2000, p. 1347-1353, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development of a Single-Reaction Multiplex PCR Toxin Typing Assay for Staphylococcus aureus Strains

Naresh K. Sharma, Catherine E. D. Rees, and Christine E. R. Dodd*

Division of Food Sciences, School of Biological Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom

Received 21 October 1999/Accepted 1 January 2000

We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the assay can detect enterotoxin genes A to E from a pure culture within 3 to 4 h. The test was used to characterize a diverse set of environmental S. aureus isolates, and a 99% correlation with toxin typing using standard immunological tests was found. The design of the assay allows it to be extended to include both newly characterized and as-yet-unknown toxin genes.

* Corresponding author. Mailing address: Division of Food Sciences, School of Biological Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom. Phone: 44(0)1159516163. Fax: 44(0)1159516162. E-mail: christine.dodd{at}nottingham.ac.uk.

Applied and Environmental Microbiology, April 2000, p. 1347-1353, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mertz, P. M., Cardenas, T. C. P., Snyder, R. V., Kinney, M. A., Davis, S. C., Plano, L. R. W. (2007). Staphylococcus aureus Virulence Factors Associated With Infected Skin Lesions: Influence on the Local Immune Response. Arch Dermatol 143: 1259-1263 [Abstract] [Full Text]  
  • Naffa, R. G., Bdour, S. M., Migdadi, H. M., Shehabi, A. A. (2006). Enterotoxicity and genetic variation among clinical Staphylococcus aureus isolates in Jordan. J Med Microbiol 55: 183-187 [Abstract] [Full Text]  
  • Carneiro, L. A.M., Queiroz, M. L.P., Merquior, V. L.C. (2004). Antimicrobial-resistance and enterotoxin-encoding genes among staphylococci isolated from expressed human breast milk. J Med Microbiol 53: 761-768 [Abstract] [Full Text]  
  • Sergeev, N., Volokhov, D., Chizhikov, V., Rasooly, A. (2004). Simultaneous Analysis of Multiple Staphylococcal Enterotoxin Genes by an Oligonucleotide Microarray Assay. J. Clin. Microbiol. 42: 2134-2143 [Abstract] [Full Text]  
  • Klotz, M., Opper, S., Heeg, K., Zimmermann, S. (2003). Detection of Staphylococcus aureus Enterotoxins A to D by Real-Time Fluorescence PCR Assay. J. Clin. Microbiol. 41: 4683-4687 [Abstract] [Full Text]  
  • Oliveira, D. C., Lencastre, H. d. (2002). Multiplex PCR Strategy for Rapid Identification of Structural Types and Variants of the mec Element in Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 46: 2155-2161 [Abstract] [Full Text]  
  • Akineden, O., Annemuller, C., Hassan, A. A., Lammler, C., Wolter, W., Zschock, M. (2001). Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis. CVI 8: 959-964 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»